製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to UXT
  • 特異性
    ab77483 is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1).
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-DHRDKVYEQLAKY

    , from an internal region of Human UXT (NP_004173.1; NP_705582.1).

  • ポジティブ・コントロール
    • Human Kidney and Skeletal Muscle lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor transcription (AR). AR N-terminus-associated coactivator which may play a role in facilitating receptor-induced transcriptional activation (PubMed:11854421). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:11827465). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). May be a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function (PubMed:17620405). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584). Together with URI1, associates with chromatin to the NKX3-1 promoter region.
  • 組織特異性
    Ubiquitous. Expressed in prostate epithelial cells. Overexpressed in a number of tumor tissues (PubMed:16221885). Highest levels in the heart, skeletal muscle, pancreas, kidney, liver, adrenal gland, peripheral blood leukocytes, lymph node, prostate, and thyroid and the lowest levels in bladder and uterus.
  • 配列類似性
    Belongs to the UXT family.
  • 細胞内局在
    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Androgen receptor trapped clone 27 protein antibody
    • ART 27 antibody
    • ART-27 antibody
    • OTTHUMP00000023226 antibody
    • OTTHUMP00000023227 antibody
    • Protein UXT antibody
    • SKP2 associated alpha PFD 1 antibody
    • STAP1 antibody
    • Ubiquitously expressed prefoldin like chaperone antibody
    • Ubiquitously expressed transcript antibody
    • Ubiquitously expressed transcript protein antibody
    • Uxt antibody
    • UXT_HUMAN antibody
    see all

画像

  • ab77483 (5µg/ml) staining of UXT in paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of cystoplasm in all epithelial cells, but nuclear staining in some of them.

  • Anti-UXT antibody (ab77483) at 0.03 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µg

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

参考文献

This product has been referenced in:
  • Hintermair C  et al. Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression. Sci Rep 6:27401 (2016). Read more (PubMed: 27264542) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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