Anti-UXT 抗体 (ab77483)
Key features and details
- Goat polyclonal to UXT
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UXT antibody
UXT 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to UXT -
由来種
Goat -
特異性
ab77483 is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1). -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Cow, Dog, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide:
C-DHRDKVYEQLAKY
, from an internal region of Human UXT (NP_004173.1; NP_705582.1). -
ポジティブ・コントロール
- Human Kidney and Skeletal Muscle lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab77483 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor transcription (AR). AR N-terminus-associated coactivator which may play a role in facilitating receptor-induced transcriptional activation (PubMed:11854421). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:11827465). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). May be a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function (PubMed:17620405). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584). Together with URI1, associates with chromatin to the NKX3-1 promoter region. -
組織特異性
Ubiquitous. Expressed in prostate epithelial cells. Overexpressed in a number of tumor tissues (PubMed:16221885). Highest levels in the heart, skeletal muscle, pancreas, kidney, liver, adrenal gland, peripheral blood leukocytes, lymph node, prostate, and thyroid and the lowest levels in bladder and uterus. -
配列類似性
Belongs to the UXT family. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 465609 Chimpanzee
- Entrez Gene: 525680 Cow
- Entrez Gene: 480900 Dog
- Entrez Gene: 8409 Human
- Omim: 300234 Human
- SwissProt: Q32P97 Cow
- SwissProt: Q9UBK9 Human
- Unigene: 172791 Human
see all -
別名
- Androgen receptor trapped clone 27 protein antibody
- ART 27 antibody
- ART-27 antibody
see all
画像
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Anti-UXT antibody (ab77483) at 0.03 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µg
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab77483 は 1 報の論文で使用されています。
- Hintermair C et al. Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression. Sci Rep 6:27401 (2016). PubMed: 27264542