製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to UTF1
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Mouse UTF1.

  • ポジティブ・コントロール
    • Mouse embryonic stem cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 22266011
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 24672748

ターゲット情報

  • 機能
    Acts as a transcriptional coactivator of ATF2.
  • ドメイン
    The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters.
  • 翻訳後修飾
    Phosphorylated.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP.
  • 別名
    • hUTF 1 antibody
    • hUTF1 antibody
    • Undifferentiated embryonic cell transcription factor 1 antibody
    • UTF 1 antibody
    • UTF1 antibody
    • UTF1_HUMAN antibody
    see all

Anti-UTF1 antibody 画像

  • ICC/IF image of ab24273 stained mouse embryonic stem cells. The cells were PFA fixed (4% PFA, 20 min) and incubated with the antibody (ab24273, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).

    The large nuclei of the feeder cells can be seen in the image; ab24273 does not localise to these nuclei. ab24273 can be seen localising to the much smaller nuclei of the mouse embryonic stem cells.

  • All lanes : Anti-UTF1 antibody (ab24273) at 1 µg/ml

    Lane 1 : Mouse Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate with Human UTF1 peptide (ab30369) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 46 kDa (possible non-specific binding,post-translational modification).

Anti-UTF1 antibody (ab24273) 使用論文

This product has been referenced in:
  • Maza I  et al. Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors. Nat Biotechnol 33:769-74 (2015). ICC/IF . Read more (PubMed: 26098448) »
  • Galonska C  et al. In Vivo and in vitro dynamics of undifferentiated embryonic cell transcription factor 1. Stem Cell Reports 2:245-52 (2014). ChIP ; Mouse . Read more (PubMed: 24672748) »

See all 6 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount
50 µg
Specification
Mouse ES Cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Dec 28 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP on chip
Sample
Mouse Cell lysate - other (male germ cell culture)
Specification
male germ cell culture
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Microarray
Positive control
The sample we used for the ChIP pulldown with this antibody was cultured mouse male germ cells with confirmed UTF1 expression by ICC. 30ug chromatin and 5ug antibody used per ChIP reaction.
Negative control
Input - gDNA from chromatin from same sample.
Username

Abcam user community

Verified customer

投稿 Jul 30 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate + 0.05% Tween-20
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse ES Cell (wt D3 line) undifferentiated)
Specification
Mouse ES Cell (wt D3 line) undifferentiated
Fixative
Paraformaldehyde
Permeabilization
Yes - see blocking buffer
Blocking step
1% serum, 0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells)
Loading amount
40 µg
Specification
Mouse Embryonic Stem Cells
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Aug 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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