Anti-Uteroglobin 抗体 (ab40873)
製品の概要
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製品名Anti-Uteroglobin antibody
Uteroglobin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Uteroglobin
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アプリケーション適用あり: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Recombinant rat uteroglobin His fusion protein.
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特記事項
This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.01% Sodium Azide
Constituents: Whole serum -
精製度Whole antiserum
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一次抗体 備考This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab40873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/1600. | |
| ELISA | Use at an assay dependent concentration. PubMed: 21787397 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 24367567 | |
| WB | 1/5000. Predicted molecular weight: 10 kDa. | |
| IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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機能Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
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組織特異性Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
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配列類似性Belongs to the secretoglobin family.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 7356 Human
- Entrez Gene: 22287 Mouse
- Entrez Gene: 25575 Rat
- Omim: 192020 Human
- SwissProt: P11684 Human
- SwissProt: Q06318 Mouse
- SwissProt: P17559 Rat
- Unigene: 523732 Human
see all -
別名
- Blastokinin antibody
- CC10 antibody
- CC16 antibody
see all
Anti-Uteroglobin antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody (ab40873)Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).
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Immunohistochemistry (Frozen sections) - Uteroglobin antibody (ab40873)Image from Nyeng P et. al., BMC Dev Biol. 2008; 8: 2 (Fig. 6D)ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image.
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Western blot - Anti-Uteroglobin antibody (ab40873)This image is courtesy of an anonymous Abreview.All lanes : Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution
Lane 1 : Mouse lung whole tissue lysate with Milk
Lane 2 : Mouse kidney whole tissue lysate with Milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 10 kDa
Exposure time : 30 secondsThis image is courtesy of an anonymous Abreview.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Uteroglobin antibody (ab40873)Image courtesy of an anonymous Abreview.ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.
プロトコール
Anti-Uteroglobin antibody (ab40873) 使用論文
This product has been referenced in:
- Strupp C et al. Relationship of Metabolism and Cell Proliferation to the Mode of Action of Fluensulfone-Induced Mouse Lung Tumors. II: Additional Mechanistic Studies. Toxicol Sci 154:296-308 (2016). Read more (PubMed: 27605420) »
- Crabbé A et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One 10:e0126846 (2015). IHC-P ; Mouse . Read more (PubMed: 25962111) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"