製品の概要

  • 製品名
    Anti-Uteroglobin antibody
    Uteroglobin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Uteroglobin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, ELISA, ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant rat uteroglobin His fusion protein.

  • 特記事項


    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    This antibody is useful for distinguishing ciliated from non-ciliated cells in the adult mouse lung.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1600.
ELISA Use at an assay dependent concentration. PubMed: 21787397
ICC/IF Use at an assay dependent concentration. PubMed: 24367567
WB 1/5000. Predicted molecular weight: 10 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
  • 組織特異性
    Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
  • 配列類似性
    Belongs to the secretoglobin family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Blastokinin antibody
    • CC10 antibody
    • CC16 antibody
    • CCPBP antibody
    • CCSP antibody
    • Clara cell phospholipid binding protein antibody
    • Clara cell phospholipid-binding protein antibody
    • Clara cell specific 10 kD protein antibody
    • Clara cells 10 kDa secretory protein antibody
    • OTTHUMP00000236107 antibody
    • SCGB1A1 antibody
    • Secretoglobin family 1A member 1 antibody
    • Secretoglobin, family 1A, member 1 (uteroglobin) antibody
    • UG antibody
    • UGB antibody
    • UP-1 antibody
    • UP1 antibody
    • Urinary protein 1 antibody
    • Urine protein 1 antibody
    • UTER_HUMAN antibody
    • Uteroglobin antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody (ab40873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody (ab40873)

    Adult mouse lung was embedded in paraffin and stained with ab40873 (1:1600 dilution).

  • Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody (ab40873)
    Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody (ab40873)Image from Nyeng P et. al., BMC Dev Biol. 2008; 8: 2 (Fig. 6D)

    ab40873 at 1/1600 dilution staining Uteroglobin in mouse embryo tissue section by Immunohistochemistry (Frozen sections). Frozen tissue slides were fixed in 4% paraformaldehyde and antigen retrieval was performed for 10 minutes in microwave oven in 0.01 M citrate buffer pH6. ab40873 stains green in proximal lung section from wild type mouse embryo as shown in image. 

  • Western blot - Anti-Uteroglobin antibody (ab40873)
    Western blot - Anti-Uteroglobin antibody (ab40873)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-Uteroglobin antibody (ab40873) at 1/5000 dilution

    Lane 1 : Mouse lung whole tissue lysate with Milk
    Lane 2 : Mouse kidney whole tissue lysate with Milk

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 10 kDa


    Exposure time: 30 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody (ab40873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody (ab40873)Image courtesy of an anonymous Abreview.
    ab40873 staining Uteroglobin in human lung parenchyma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer. Samples were then blocked with 1% BSA, 0.1% Triton-X/ PBS for 15 minutes at 18°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An HRP-conjugated pig anti-rabbit polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Strupp C  et al. Relationship of Metabolism and Cell Proliferation to the Mode of Action of Fluensulfone-Induced Mouse Lung Tumors. II: Additional Mechanistic Studies. Toxicol Sci 154:296-308 (2016). Read more (PubMed: 27605420) »
  • Cloonan SM  et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22:163-74 (2016). Read more (PubMed: 26752519) »

See all 16 Publications for this product

ab40873 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

6 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uteroglobin antibody

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Permeabilization
Yes - Triton X-100 0.1%
Specification
adult lung
Fixative
Formaldehyde
Read More
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Mrs. Agnes Francois

Verified customer

投稿 Dec 11 2017

Western blot abreview for Anti-Uteroglobin antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
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Abcam user community

Verified customer

投稿 Feb 20 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Uteroglobin antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, bronchial epithelium)
Permeabilization
No
Specification
lung, bronchial epithelium
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
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Username

Abcam user community

Verified customer

投稿 Dec 14 2016

Western blot abreview for Anti-Uteroglobin antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (urine)
Loading amount
5 µg
Specification
urine
Treatment
CLB (NP-40 based lysis buffer with protease inhibitors) , 10 min on ice
Gel Running Conditions
Reduced Denaturing (18)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
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Username

Abcam user community

Verified customer

投稿 Nov 23 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uteroglobin antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Lung Parenchyma)
Specification
Human Lung Parenchyma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
1% BSA with 0.1% Triton-X in PBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 18°C
Read More
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Username

Abcam user community

Verified customer

投稿 Mar 15 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uteroglobin antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Trypsin (Ab970) 0.37%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Sep 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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