Anti-USP48 抗体 (ab72226)
Key features and details
- Rabbit polyclonal to USP48
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP48 antibody
USP48 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP48 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to a region between residues 985 and 1035 of human USP48 (NP_115612.4).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72226 was affinity purified using an epitope specific to USP48 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72226の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (4) |
1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 119 kDa).
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IP | (1) |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 119 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the peptidase C19 family.
Contains 3 DUSP domains.
Contains 1 ubiquitin-like domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 769457 Chicken
- Entrez Gene: 84196 Human
- Entrez Gene: 170707 Mouse
- Entrez Gene: 362636 Rat
- SwissProt: Q5ZM45 Chicken
- SwissProt: Q86UV5 Human
- SwissProt: Q3V0C5 Mouse
- SwissProt: Q76LT8 Rat
see all -
別名
- AAH67261 antibody
- Deubiquitinating enzyme 48 antibody
- DKFZp762M1713 antibody
see all
画像
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Immunocytochemistry analysis of paraformaldehyde-fixed human ARPE-19 cells permeabilized with 0.5% Triton X-100 for 20 minutes at RT, staining with ab72226 at 1/50 dilution. Secondary antibody was Alexa Fluor™ 488 goat anti-rabbit IgG at 1/300 dilution. Cells were incubated with the primary antibody for 12 hours at 4deg;C. Blocking was done with 3% BSA for 1 hour at 25°C.
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All lanes : Anti-USP48 antibody (ab72226) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 119 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
Detection of USP48 by Western Blot of Immunprecipitate.
ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab72226 は 7 報の論文で使用されています。
- Faulkner EL et al. Imaging nanoscale nuclear structures with expansion microscopy. J Cell Sci 135:N/A (2022). PubMed: 35748225
- Jantaree P et al. USP48 and A20 synergistically promote cell survival in Helicobacter pylori infection. Cell Mol Life Sci 79:461 (2022). PubMed: 35913642
- Sánchez-Bellver L et al. The Deubiquitinating Enzyme USP48 Interacts with the Retinal Degeneration-Associated Proteins UNC119a and ARL3. Int J Mol Sci 23:N/A (2022). PubMed: 36293380
- Chen Y & Ding Y LINC00467 enhances head and neck squamous cell carcinoma progression and the epithelial-mesenchymal transition process via miR-299-5p/ubiquitin specific protease-48 axis. J Gene Med 22:e3184 (2020). PubMed: 32159247
- Ghanem A et al. Catalytic domain of deubiquitinylase USP48 directs interaction with Rel homology domain of nuclear factor kappaB transcription factor RelA. Mol Biol Rep 46:1369-1375 (2019). PubMed: 30628021
- Li L et al. Deubiquitinase USP48 promotes ATRA-induced granulocytic differentiation of acute promyelocytic leukemia cells. Int J Oncol 53:895-903 (2018). PubMed: 29901102
- Zhou A et al. Gli1-induced deubiquitinase USP48 aids glioblastoma tumorigenesis by stabilizing Gli1. EMBO Rep 18:1318-1330 (2017). PubMed: 28623188