製品の概要
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製品名Anti-USP48 antibody
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製品の詳細Rabbit polyclonal to USP48
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WB, IPmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to a region between residues 985 and 1035 of human USP48 (NP_115612.4).
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ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab72226 was affinity purified using an epitope specific to USP48 immobilized on solid support.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab72226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 119 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
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組織特異性Widely expressed.
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配列類似性Belongs to the peptidase C19 family.
Contains 3 DUSP domains.
Contains 1 ubiquitin-like domain. -
細胞内局在Cytoplasm. Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 769457 Chicken
- Entrez Gene: 84196 Human
- Entrez Gene: 170707 Mouse
- Entrez Gene: 362636 Rat
- SwissProt: Q5ZM45 Chicken
- SwissProt: Q86UV5 Human
- SwissProt: Q3V0C5 Mouse
- SwissProt: Q76LT8 Rat
see all -
別名
- AAH67261 antibody
- Deubiquitinating enzyme 48 antibody
- DKFZp762M1713 antibody
see all
画像
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Western blot - USP48 antibody (ab72226)All lanes : Anti-USP48 antibody (ab72226) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 119 kDa
Observed band size: 145 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 seconds -
Immunoprecipitation - USP48 antibody (ab72226)Detection of USP48 by Western Blot of Immunprecipitate.
ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second. -
Immunocytochemistry/ Immunofluorescence-Anti-USP48 antibody(ab72226)ICC/IF image of ab72226 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
ab72226 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"