製品の概要

  • 製品名
    Anti-USP48 antibody
  • 製品の詳細
    Rabbit polyclonal to USP48
  • アプリケーション
    適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residues 985 and 1035 of human USP48 (NP_115612.4).

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 119 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the peptidase C19 family.
    Contains 3 DUSP domains.
    Contains 1 ubiquitin-like domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAH67261 antibody
    • Deubiquitinating enzyme 48 antibody
    • DKFZp762M1713 antibody
    • EC 3.1.2.15 antibody
    • Kiaa4202 antibody
    • MGC132556 antibody
    • MGC14879 antibody
    • RAP1GA1 antibody
    • Synaptic ubiquitin specific protease antibody
    • synUSP antibody
    • Ubiquitin carboxyl terminal hydrolase 48 antibody
    • Ubiquitin carboxyl-terminal hydrolase 48 antibody
    • Ubiquitin specific peptidase 48 antibody
    • Ubiquitin specific processing protease 48 antibody
    • Ubiquitin specific protease 31 antibody
    • Ubiquitin specific protease 48 antibody
    • Ubiquitin thioesterase 48 antibody
    • Ubiquitin thiolesterase 48 antibody
    • Ubiquitin-specific-processing protease 48 antibody
    • UBP48_HUMAN antibody
    • USP31 antibody
    • USP48 antibody
    see all

画像

  • All lanes : Anti-USP48 antibody (ab72226) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 119 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds
  • Detection of USP48 by Western Blot of Immunprecipitate.
    ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 1 second.
  • ICC/IF image of ab72226 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab72226 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Specification
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Dec 22 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HaCaT cells)
Specification
HaCaT cells
Treatment
USP48 siRNA (300pM per 10 cm dish) for 48 hours or control treated
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Oct 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Hela Cells)
Loading amount
50 µg
Specification
Hela Cells
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Verified customer

投稿 Nov 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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