Anti-USP47 抗体 (ab72143)
Key features and details
- Rabbit polyclonal to USP47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP47 antibody
USP47 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP47 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to a region between residues 1 and 50 of human USP47 (NP_060414.3)
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72143の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 158 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Putative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues. -
組織特異性
Expressed in skeletal muscle, heart and testis. -
配列類似性
Belongs to the peptidase C19 family. - Information by UniProt
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参照データベース
- Entrez Gene: 55031 Human
- Entrez Gene: 74996 Mouse
- Omim: 614460 Human
- SwissProt: Q96K76 Human
- SwissProt: Q8BY87 Mouse
- Unigene: 577256 Human
- Unigene: 16974 Mouse
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別名
- Deubiquitinating enzyme 47 antibody
- TFRP antibody
- Trf TATA binding protein related factor proximal homolog antibody
see all
画像
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All lanes : Anti-USP47 antibody (ab72143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 158 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab72143 は 5 報の論文で使用されています。
- Yuan F et al. Long non-coding RNA KCNQ1OT1 promotes nasopharyngeal carcinoma cell cisplatin resistance via the miR-454/USP47 axis. Int J Mol Med 47:N/A (2021). PubMed: 33576460
- Hu Y et al. USP47 promotes apoptosis in rat myocardial cells after ischemia/reperfusion injury via NF-κB activation. Biotechnol Appl Biochem 68:841-848 (2021). PubMed: 32761659
- Guo X et al. E2F7 Transcriptionally Inhibits MicroRNA-199b Expression to Promote USP47, Thereby Enhancing Colon Cancer Tumor Stem Cell Activity and Promoting the Occurrence of Colon Cancer. Front Oncol 10:565449 (2020). PubMed: 33489876
- Yan S et al. LINC00668 promotes tumorigenesis and progression through sponging miR-188-5p and regulating USP47 in colorectal cancer. Eur J Pharmacol 858:172464 (2019). PubMed: 31233752
- Sako-Kubota K et al. Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions. Mol Biol Cell 25:3851-60 (2014). PubMed: 25253721