製品の概要

  • 製品名
    Anti-USP40 antibody
  • 製品の詳細
    Rabbit polyclonal to USP40
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 755-900 of Human USP40.

  • ポジティブ・コントロール
    • Human tonsil tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use Buffer at pH 6 for antigen retrieval.

ターゲット情報

  • 機能
    May be catalytically inactive.
  • 組織特異性
    Broadly expressed.
  • 配列類似性
    Belongs to the peptidase C19 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme 40 antibody
    • Ubiquitin carboxyl-terminal hydrolase 40 antibody
    • Ubiquitin thiolesterase 40 antibody
    • Ubiquitin-specific-processing protease 40 antibody
    • UBP40_HUMAN antibody
    • Usp40 antibody
    see all

Anti-USP40 antibody 画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles and focal adhesions. Recommended concentration of ab121234 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121234, at 1/75 dilution, staining USP40 in Human tonsil tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

    Note: shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.

Anti-USP40 antibody (ab121234) 使用論文

ab121234 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - other (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jan 06 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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