製品の概要

  • 製品名
    Anti-USP37 antibody
    USP37 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to USP37
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids 930-979 of Human USP37 (NP_065986.1)

  • ポジティブ･コントロール
    • HeLa whole cell lysate 239T whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 110 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性
    Expressed in brain and prostate.
  • 配列類似性
    Belongs to the peptidase C19 family.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Deubiquitinating enzyme 3 antibody
      • Deubiquitinating enzyme 37 antibody
      • EC 3.1.2.15 antibody
      • KIAA1594 antibody
      • MGC117261 antibody
      • OTTHUMP00000164146 antibody
      • tmp_locus_50 antibody
      • Ubiquitin carboxyl-terminal hydrolase 37 antibody
      • Ubiquitin specific peptidase 37 antibody
      • Ubiquitin specific processing protease 37 antibody
      • Ubiquitin specific protease 37 antibody
      • Ubiquitin thioesterase 37 antibody
      • Ubiquitin thiolesterase 37 antibody
      • Ubiquitin-specific-processing protease 37 antibody
      • UBP37_HUMAN antibody
      • USP37 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP37 antibody (ab72199)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP37 antibody (ab72199)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling USP37 with ab72199 at 1/200 (1µg/ml). Detection: DAB.
    • Western blot - Anti-USP37 antibody (ab72199)
      Western blot - Anti-USP37 antibody (ab72199)
      All lanes : Anti-USP37 antibody (ab72199) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 110 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 95-117 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-USP37 antibody (ab72199)
      Immunoprecipitation - Anti-USP37 antibody (ab72199)
      Immunoprecipitation of HeLa whole cell lysate (1mg) using ab72199 at 3µg/mg lysate. 20% of the immunoprecipitate was used for Western blotting and detected using ab72199 at 1µg/ml. Bands were exposed by chemiluminescence with an exposure time of 10 seconds.

    参考文献

    ab72199 has not yet been referenced specifically in any publications.

    ab72199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    Western blot abreview for Anti-USP37 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8% Tris-Glycine)
    Sample
    Human Cell lysate - whole cell (Osteoblast)
    Specification
    Osteoblast
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    abreview image
    Username

    Dr. Carlos le Sage

    Verified customer

    投稿 Aug 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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