製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to USP35
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Dog, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 967 and 1017 of human USP35 (CAE51937.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 113 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性
      Expressed in testis, pancreas and skeletal muscle.
    • 配列類似性
      Belongs to the peptidase C19 family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Deubiquitinating enzyme 35 antibody
      • Gm1088 antibody
      • Gm493 antibody
      • KIAA1372 antibody
      • Ubiquitin carboxyl terminal hydrolase 35 antibody
      • Ubiquitin carboxyl-terminal hydrolase 35 antibody
      • Ubiquitin specific peptidase 35 antibody
      • Ubiquitin specific processing protease 35 antibody
      • Ubiquitin specific protease 35 antibody
      • Ubiquitin thioesterase 35 antibody
      • Ubiquitin thiolesterase 35 antibody
      • Ubiquitin-specific-processing protease 35 antibody
      • UBP35_HUMAN antibody
      • USP34 antibody
      • USP35 antibody
      see all

    画像

    • All lanes : Anti-USP35 antibody (ab86791) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 113 kDa
      Observed band size : 113 kDa


      Exposure time : 3 minutes
    • Detection of USP35 by Western Blot of Immunprecipitate.
      ab86791 at 1 µg/ml staining USP35 in HeLa whole cell lysate immunoprecipitated using ab86791 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    参考文献

    This product has been referenced in:
    • Liu C  et al. USP35 activated by miR let-7a inhibits cell proliferation and NF-?B activation through stabilization of ABIN-2. Oncotarget 6:27891-906 (2015). Read more (PubMed: 26348204) »
    • Zhang Y  et al. Effects of 1.8 GHz radiofrequency radiation on protein expression in human lens epithelial cells. Hum Exp Toxicol N/A:N/A (2013). WB ; Human . Read more (PubMed: 23338683) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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