All lanes : Anti-USP32 antibody (ab86792) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 182 kDa Observed band size : 182 kDa Additional bands at : 150 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunoprecipitation - USP32 antibody (ab86792)
Detection of USP32 by Western Blot of Immunprecipitate.
ab86792, at 1 µg/ml, staining USP32 in HeLa whole cell lysate immunoprecipitated using ab86792 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.