Anti-USP3 抗体 (ab80597)
Key features and details
- Rabbit polyclonal to USP3
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP3 antibody
USP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla -
免疫原
Synthetic peptide (Human) mapped to a region between residues 471 and 521
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ポジティブ・コントロール
- IP: HeLa whole cell lysate; IHC: human ovarian carcinoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.5% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin. -
組織特異性
Expressed in all tissues examined, with strongest expression in pancreas. -
配列類似性
Belongs to the peptidase C19 family. USP3 subfamily.
Contains 1 UBP-type zinc finger. -
ドメイン
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination. -
細胞内局在
Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 9960 Human
- GenBank: NM_006537 Human
- Omim: 604728 Human
- SwissProt: Q9Y6I4 Human
- Unigene: 458499 Human
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別名
- Deubiquitinating enzyme 3 antibody
- EC 3.1.2.15 antibody
- SIH003 antibody
see all
画像
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USP3 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab80597 at 3 µg per mg lysate. Western blot was performed on the immunoprecipitate using ab80597 at 1.0 µg/mL.
Lane 1: ab80597 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP3 with ab80597 at 1/1000 (1µg/ml). Detection: DAB.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab80597 は 1 報の論文で使用されています。
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835