Anti-USP28 抗体 [EPR4250(2)] (ab110744)


  • 製品名Anti-USP28 antibody [EPR4250(2)]
    USP28 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4250(2)] to USP28
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human USP28.

  • ポジティブ・コントロール
    • 293T, SW480, HeLa, and A431 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.




Our Abpromise guarantee covers the use of ab110744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
    • 配列類似性Belongs to the peptidase C19 family. USP28 subfamily.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • 翻訳後修飾Degradaded upon nickel ion level or hypoxia exposure.
      Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
    • 細胞内局在Nucleus > nucleoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Deubiquitinating enzyme 28 antibody
      • KIAA1515 antibody
      • Ubiquitin carboxyl terminal hydrolase 28 antibody
      • Ubiquitin carboxyl-terminal hydrolase 28 antibody
      • ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
      • Ubiquitin specific peptidase 28 antibody
      • Ubiquitin specific processing protease 28 antibody
      • Ubiquitin specific protease 28 antibody
      • Ubiquitin thioesterase 28 antibody
      • Ubiquitin thiolesterase 28 antibody
      • Ubiquitin-specific-processing protease 28 antibody
      • UBP28_HUMAN antibody
      • USP 28 antibody
      • USP28 antibody
      • USP28 protein antibody
      see all

    Anti-USP28 antibody [EPR4250(2)] 画像

    • Predicted band size : 122 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE.  Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 122 kDa

    Anti-USP28 antibody [EPR4250(2)] (ab110744) 使用論文

    ab110744 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Xenopus laevis Cell lysate - whole cell (egg extracts)
    Specification egg extracts
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

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    投稿 Sep 02 2014