製品の概要

  • 製品名Anti-USP24 antibody
    USP24 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to USP24
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide from a region between residue 2410 and the C-terminus (residue 2460) of human USP24 (XP_371254.5)

  • ポジティブ・コントロール
    • Whole cell lysate from Hela and 293T cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/2,000 - 1/10,000. Detects bands of approximately 71 kDa and 250 kDa (predicted molecular weight: 211 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    Anti-USP24 antibody 画像

    • All lanes : Anti-USP24 antibody (ab72241) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 211 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of USP24.
      Lane 1: ab72241 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72241 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    Anti-USP24 antibody (ab72241) 使用論文

    ab72241 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vi...

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    Thank you for contacting Abcam regarding our USP24 antibodies.


    Regarding the difference in molecular weight of the bands recognized by these antibodies:


    ab72241 - The WB image on our datasheet is representative of endogenous...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"