製品の概要

  • 製品名Anti-USP22 antibody
    USP22 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to USP22
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 71-120 (RLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIY D) of Human USP22 (NP_056091)

  • ポジティブ・コントロール
    • Fetal heart lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
  • 組織特異性Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
  • 配列類似性Belongs to the peptidase C19 family. UBP8 subfamily.
    Contains 1 UBP-type zinc finger.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme 22 antibody
    • KIAA1063 antibody
    • Ubiquitin carboxyl terminal hydrolase 22 antibody
    • Ubiquitin carboxyl-terminal hydrolase 22 antibody
    • Ubiquitin specific peptidase 22 antibody
    • Ubiquitin specific peptidase 3 like antibody
    • Ubiquitin specific processing protease 22 antibody
    • Ubiquitin specific protease 22 antibody
    • Ubiquitin thioesterase 22 antibody
    • Ubiquitin thiolesterase 22 antibody
    • Ubiquitin-specific-processing protease 22 antibody
    • UBP22_HUMAN antibody
    • USP 22 antibody
    • Usp22 antibody
    • USP3L antibody
    see all

Anti-USP22 antibody 画像

  • Anti-USP22 antibody (ab82986) at 1 µg/ml + Fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-USP22 antibody (ab82986) 使用論文

ab82986 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Aug 15 2016

Application Western blot
Sample Zebrafish Cell lysate - whole cell (Whole embryo)
Gel Running Conditions Reduced Denaturing (10% Bis-Tris gel with MES running buffer)
Loading amount 60 µg
Specification Whole embryo
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Stephen Moore

Verified customer

投稿 Jun 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"