製品の概要

  • 製品名Anti-USP20 antibody
    USP20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to USP20
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Chicken, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 863 and 913 of human USP20 (NP_006667.2).

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • 配列類似性Belongs to the peptidase C19 family. USP20/USP33 subfamily.
    Contains 2 DUSP domains.
    Contains 1 UBP-type zinc finger.
  • ドメインThe UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
  • 翻訳後修飾Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
  • 細胞内局在Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme 20 antibody
    • hVDU2 antibody
    • KIAA1003 antibody
    • LSFR 3A antibody
    • LSFR3A antibody
    • pVHL interacting deubiquitinating enzyme 2 antibody
    • Ubiquitin carboxyl terminal hydrolase 20 antibody
    • Ubiquitin carboxyl-terminal hydrolase 20 antibody
    • ubiquitin specific peptidase 20 antibody
    • Ubiquitin specific processing protease 20 antibody
    • Ubiquitin specific protease 20 antibody
    • Ubiquitin thioesterase 20 antibody
    • Ubiquitin thiolesterase 20 antibody
    • Ubiquitin-specific-processing protease 20 antibody
    • UBP20_HUMAN antibody
    • USP 20 antibody
    • USP20 antibody
    • VDU 2 antibody
    • VDU2 antibody
    • VHL interacting deubiquitinating enzyme 2 antibody
    • VHL-interacting deubiquitinating enzyme 2 antibody
    see all

Anti-USP20 antibody 画像

  • All lanes : Anti-USP20 antibody (ab72225) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 102 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of USP20 by Western Blot of Immunprecipitate.
    ab72225 at 1µg/ml staining USP20 in HeLa whole cell lysate immunoprecipitated using ab72225 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-USP20 antibody (ab72225) 使用論文

ab72225 has not yet been referenced specifically in any publications.

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