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RabMAb

Anti-USP13 抗体 [EPR4348] (ab109264)

製品の概要

  • 製品名
    Anti-USP13 antibody [EPR4348]
    USP13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4348] to USP13
  • アプリケーション
    適用あり: WB, IP, ICC, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to Human USP13.

  • ポジティブ・コントロール
    • SH SY5Y, HepG2 and A375 cell lysates This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/940 - 1/9400. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 組織特異性
      Highly expressed in ovary and testes.
    • 配列類似性
      Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Deubiquitinating enzyme 13 antibody
      • Isopeptidase T 3 antibody
      • Isopeptidase T-3 antibody
      • Isopeptidase T3 antibody
      • ISOT 3 antibody
      • ISOT-3 antibody
      • ISOT3 antibody
      • Ubiquitin carboxyl terminal hydrolase 13 antibody
      • Ubiquitin carboxyl-terminal hydrolase 13 antibody
      • Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
      • Ubiquitin specific peptidase 13 antibody
      • Ubiquitin specific processing protease 13 antibody
      • Ubiquitin specific protease 13 antibody
      • Ubiquitin thioesterase 13 antibody
      • Ubiquitin thiolesterase 13 antibody
      • Ubiquitin-specific-processing protease 13 antibody
      • UBP13 antibody
      • UBP13_HUMAN antibody
      • USP 13 antibody
      • USP13 antibody
      see all

    Anti-USP13 antibody [EPR4348] 画像

    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (Mouse neuroblastoma cell line) labeling USP13 with Purified ab109264 at 1/500 dilution (5 µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.



    • Predicted band size : 97 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP13 knockout HAP1 cell lysate (20 µg)
      Lane 3: A375 cell lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109264 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109264 was shown to recognize USP13 when USP13 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP13 knockout samples were subjected to SDS-PAGE. ab109264 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing SHSY-5Y cells stained with ab109264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109264, 1/9400 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • ICC/IF image of ab109264 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109264 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-USP13 antibody [EPR4348] (ab109264) at 1/1000 dilution

      Lane 1 : SH SY5Y cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 97 kDa
      Observed band size : 97 kDa

    Anti-USP13 antibody [EPR4348] (ab109264) 使用論文

    ab109264 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (4-12 % Bis-Tris gel)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 10 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Non-reduced Denaturing (10%SDS)
    Loading amount
    80 µg
    Specification
    293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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