Anti-USP13 抗体 [EPR4348] (ab109264)

製品の概要

  • 製品名Anti-USP13 antibody [EPR4348]
    USP13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4348] to USP13
  • アプリケーション適用あり: WB, IP, ICC, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to Human USP13.

  • ポジティブ・コントロール
    • SH SY5Y, HepG2 and A375 cell lysates This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/940 - 1/9400. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 組織特異性Highly expressed in ovary and testes.
    • 配列類似性Belongs to the peptidase C19 family.
      Contains 2 UBA domains.
      Contains 1 UBP-type zinc finger.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Deubiquitinating enzyme 13 antibody
      • Isopeptidase T 3 antibody
      • Isopeptidase T-3 antibody
      • Isopeptidase T3 antibody
      • ISOT 3 antibody
      • ISOT-3 antibody
      • ISOT3 antibody
      • Ubiquitin carboxyl terminal hydrolase 13 antibody
      • Ubiquitin carboxyl-terminal hydrolase 13 antibody
      • Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
      • Ubiquitin specific peptidase 13 antibody
      • Ubiquitin specific processing protease 13 antibody
      • Ubiquitin specific protease 13 antibody
      • Ubiquitin thioesterase 13 antibody
      • Ubiquitin thiolesterase 13 antibody
      • Ubiquitin-specific-processing protease 13 antibody
      • UBP13 antibody
      • UBP13_HUMAN antibody
      • USP 13 antibody
      • USP13 antibody
      see all

    Anti-USP13 antibody [EPR4348] 画像



    • Predicted band size : 97 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP13 knockout HAP1 cell lysate (20 µg)
      Lane 3: A375 cell lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109264 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109264 was shown to recognize USP13 when USP13 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP13 knockout samples were subjected to SDS-PAGE. ab109264 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing SHSY-5Y cells stained with ab109264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109264, 1/9400 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • ICC/IF image of ab109264 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109264 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-USP13 antibody [EPR4348] (ab109264) at 1/1000 dilution

      Lane 1 : SH SY5Y cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 97 kDa
      Observed band size : 97 kDa

    Anti-USP13 antibody [EPR4348] (ab109264) 使用論文

    ab109264 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions Non-reduced Denaturing (4-12 % Bis-Tris gel)
    Loading amount 50 µg
    Specification HAP1 cell line
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 10 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293T)
    Gel Running Conditions Non-reduced Denaturing (10%SDS)
    Loading amount 80 µg
    Specification 293T
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"