製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to USP1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 701-750 (SETSDTTGTH ESDRNKESSD QTGINISGFE NKISYVVQSL KEYEGKWLLF) of Human USP1 (NP_003359).

  • ポジティブ・コントロール
    • Human fetal brain lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
  • 配列類似性
    Belongs to the peptidase C19 family.
  • 発生段階
    Cell cycle-regulated. Highest level during S phase.
  • 翻訳後修飾
    Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
    Ubiquitinated; leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme 1 antibody
    • hUBP antibody
    • Ubiquitin carboxyl terminal hydrolase 1 antibody
    • Ubiquitin carboxyl-terminal hydrolase 1 antibody
    • Ubiquitin specific peptidase 1 antibody
    • Ubiquitin specific protease 1 antibody
    • Ubiquitin thioesterase 1 antibody
    • Ubiquitin thiolesterase 1 antibody
    • Ubiquitin-specific-processing protease 1 antibody
    • UBP antibody
    • UBP1_HUMAN antibody
    • USP 1 antibody
    • Usp1 antibody
    see all

画像



  • Predicted band size : 88 kDa
    Western blot analysis of 293T cell lysate labeling USP1 with ab108104 at 1.0µg/ml.
  • Anti-USP1 antibody (ab108104) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size : 88 kDa
    Gel concentration: 12%.

プロトコール

参考文献

This product has been referenced in:
  • Yoon JH  et al. PAR-CLIP analysis uncovers AUF1 impact on target RNA fate and genome integrity. Nat Commun 5:5248 (2014). WB ; Human . Read more (PubMed: 25366541) »
  • Liang Q  et al. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol 10:298-304 (2014). Read more (PubMed: 24531842) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (cultured primary NK cells)
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris, MES buffers)
Loading amount
800000 cells
Treatment
expanded in IL15
Specification
cultured primary NK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Lynn Corcoran

Verified customer

投稿 Jun 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
20 µg
Specification
HEK293 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Aug 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録