製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHb12 antibody
    • Class B basic helix-loop-helix protein 12 antibody
    • FIP antibody
    • FOS interacting protein antibody
    • FOS-interacting protein antibody
    • Major late transcription factor 2 antibody
    • Upstream stimulatory factor 2 antibody
    • Upstream transcription factor 2 antibody
    • USF 2 antibody
    • Usf2 antibody
    • USF2_HUMAN antibody
    see all

画像

  • All lanes : Anti-USF2 antibody (ab125184) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 37 kDa


    Exposure time : 30 seconds
  • ab125184, at 6 µg/mg lysate, detecting USF2 in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125184 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 10 seconds.

参考文献

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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