Anti-Uroplakin III 抗体 [SFI-1] (ab78196)
Key features and details
- Mouse monoclonal [SFI-1] to Uroplakin III
- Suitable for: IHC-P
- Reacts with: Rat, Human, Common marmoset
- Isotype: IgG1
製品の概要
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製品名
Anti-Uroplakin III antibody [SFI-1]
Uroplakin III 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SFI-1] to Uroplakin III -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rat, Human, Common marmoset -
免疫原
Synthetic peptide corresponding to an internal human Uroplakin III.
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ポジティブ・コントロール
- Urothelial tissue, bladder.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Proclin 300
Constituents: 1% BSA, 0.58% Sodium chloride, 0.1% Tween, 0.363% Tris
Containing antibody stabilizer -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
SFI-1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (7) |
1/20 - 1/30. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate 30 minutes at room temperature.
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特記事項 |
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IHC-P
1/20 - 1/30. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate 30 minutes at room temperature. |
ターゲット情報
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機能
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. -
組織特異性
Expressed in ureter. -
関連疾患
Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. -
配列類似性
Belongs to the uroplakin-3 family. -
細胞内局在
Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). - Information by UniProt
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参照データベース
- Entrez Gene: 7380 Human
- Entrez Gene: 315190 Rat
- Omim: 611559 Human
- SwissProt: O75631 Human
- Unigene: 632787 Human
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別名
- MGC119178 antibody
- OTTHUMP00000028951 antibody
- UP III antibody
see all
画像
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ab78196 staining Uroplakin III in mouse bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/600 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Positivity of only the lumenal cytoplasm of the Umbrella cells is clear.
As this is a mouse primary antibody, endogenous IgG will also be visualised in the interstitial spaces (red arrowheads), in Plasma cells (black arrowheads) and also in blood vessels if the animal has not been perfused-washed/fixed. -
ab78196 staining Uroplakin III in cow ureter tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Image shows clear, strong labelling of the lumenal membranes of the Umbrella cells.
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IHC-P image of Uroplakin-III staining on Marmoset bladder sections using ab78196 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab78196 was diluetd 1:400 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to anti mouse IgG conjugated to biotin (1:200)
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IHC-P image of Uroplakin-III staining on Rat bladder sections using ab78196 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab78196 was diluetd 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to anti mouse IgG conjugated to biotin (1:250)
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ab78196 at 1/20 dilution staining Uroplakin III in human bladder by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab78196 は 9 報の論文で使用されています。
- Brossard C et al. Mesenchymal stem cells limit vascular and epithelial damage and restore the impermeability of the urothelium in chronic radiation cystitis. Stem Cell Res Ther 14:5 (2023). PubMed: 36627674
- Yao R et al. Polysaccharides from Vaccaria segetalis seeds reduce urinary tract infections by inhibiting the adhesion and invasion abilities of uropathogenic Escherichia coli. Front Cell Infect Microbiol 12:1004751 (2022). PubMed: 36506014
- Zhang XZ et al. Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model. Bioact Mater 6:1827-1838 (2021). PubMed: 33336114
- Lin KL et al. Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial. Int J Mol Sci 22:N/A (2021). PubMed: 34502202
- Zwaans BMM et al. Voiding defects in acute radiation cystitis driven by urothelial barrier defect through loss of E-cadherin, ZO-1 and Uroplakin III. Sci Rep 11:19277 (2021). PubMed: 34588475
- Fan G et al. miR-33a hinders the differentiation of adipose mesenchymal stem cells towards urothelial cells in an inductive condition by targeting ß-catenin and TGFR. Mol Med Rep 17:2341-2348 (2018). PubMed: 29207162
- Hurst CD et al. Genomic Subtypes of Non-invasive Bladder Cancer with Distinct Metabolic Profile and Female Gender Bias in KDM6A Mutation Frequency. Cancer Cell 32:701-715.e7 (2017). PubMed: 29136510
- Liu KM et al. Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum. Am J Physiol Renal Physiol 309:F318-31 (2015). WB ; Rat . PubMed: 26109091
- Wu S et al. Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. Biomaterials 32:1317-26 (2011). IHC-Fr ; Human . PubMed: 21055807