製品の概要

  • 製品名
    Anti-Uroplakin III antibody [SFI-1]
    Uroplakin III 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SFI-1] to Uroplakin III
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Common marmoset
  • 免疫原

    Synthetic peptide corresponding to an internal human Uroplakin III.

  • ポジティブ・コントロール
    • Urothelial tissue, bladder.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Proclin
    Constituents: 1% BSA, 0.1% Tween 20, 30mM Tris, 100mM Sodium chloride
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    SFI-1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 21055807
IHC-P 1/20 - 1/30. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate 30 minutes at room temperature.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

ターゲット情報

  • 機能
    Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence.
  • 組織特異性
    Expressed in ureter.
  • 関連疾患
    Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
  • 配列類似性
    Belongs to the uroplakin-3 family.
  • 細胞内局在
    Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER).
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC119178 antibody
    • OTTHUMP00000028951 antibody
    • UP III antibody
    • UP3a antibody
    • UPIII antibody
    • UPIIIA antibody
    • UPK 3 antibody
    • UPK 3A antibody
    • UPK3 antibody
    • UPK3A antibody
    • UPK3A_HUMAN antibody
    • Uroplakin 3A antibody
    • Uroplakin III antibody
    • Uroplakin-3a antibody
    • Uroplakin3A antibody
    • UroplakinIII antibody
    see all

Anti-Uroplakin III antibody [SFI-1] 画像

  • ab78196 staining Uroplakin III in mouse bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/600 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    Positivity of only the lumenal cytoplasm of the Umbrella cells is clear.
    As this is a mouse primary antibody, endogenous IgG will also be visualised in the interstitial spaces (red arrowheads), in Plasma cells (black arrowheads) and also in blood vessels if the animal has not been perfused-washed/fixed.

    See Abreview

  • ab78196 staining Uroplakin III in cow ureter tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    Image shows clear, strong labelling of the lumenal membranes of the Umbrella cells.

    See Abreview

  • IHC-P image of Uroplakin-III staining on Marmoset bladder sections using ab78196 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab78196 was diluetd 1:400 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to anti mouse IgG conjugated to biotin (1:200)

     

  • IHC-P image of Uroplakin-III staining on Rat bladder sections using ab78196 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab78196 was diluetd 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to anti mouse IgG conjugated to biotin (1:250)

  • ab78196 at 1/20 dilution staining Uroplakin III in human bladder by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Anti-Uroplakin III antibody [SFI-1] (ab78196) 使用論文

This product has been referenced in:
  • Liu KM  et al. Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum. Am J Physiol Renal Physiol 309:F318-31 (2015). WB ; Rat . Read more (PubMed: 26109091) »
  • Wu S  et al. Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. Biomaterials 32:1317-26 (2011). IHC-Fr ; Human . Read more (PubMed: 21055807) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bladder)
Permeabilization
Yes - Triton 0.1%
Specification
Bladder
Blocking step
CASBlock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Ureter)
Specification
Ureter
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jun 02 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Cat Tissue sections (Ureter)
Specification
Ureter
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 May 30 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Cow Tissue sections (Ureter)
Specification
Ureter
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 06 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Marmoset (common) Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 05 2013

Thank you for your reply. It is possible that this band may be non-specific, or it may represent Uroplakin III in a different form. Forexample, if the protein is heavily glycosylated it may run higher than predicted. Alternatively, if isoform 2 of Urop...

Read More

Thank you for contacting us. According to the uniprot database (linked below), the predicted molecular weight of the Uroplakin III precursor, isoform 1, is 30.6 kDa. After the removal of the 18 amino acid signal peptide and the additional weight from...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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