製品の概要

  • 製品名
    Anti-Uroplakin Ib antibody
  • 製品の詳細
    Rabbit polyclonal to Uroplakin Ib
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 166-196 of Human Uroplakin Ib

  • ポジティブ・コントロール
    • K562 cell lysate, Human Brain Tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
  • 組織特異性
    Bladder epithelium.
  • 配列類似性
    Belongs to the tetraspanin (TM4SF) family.
  • 翻訳後修飾
    N-glycosylated with high-mannose oligosaccharides.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tetraspanin-20 antibody
    • Tspan-20 antibody
    • TSPAN20 antibody
    • UP1b antibody
    • UPIb antibody
    • UPK1B antibody
    • UPK1B_HUMAN antibody
    • Uroplakin Ib antibody
    • Uroplakin-1b antibody
    see all

画像

  • Anti-Uroplakin Ib antibody (ab102961) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 30 kDa
  • Immunohistochemical staining of formalin fixed and paraffin embedded brain tissue followed by HRP-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells (green histogram) using ab102961 compared to a negative control cell (blue histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

参考文献

ab102961 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (gingiva)
Specification
gingiva
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録