The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 49 kDa. Approx 48kDa band observed in lysates of Human Kidney fibroblast cell line HEK293. A minor band was also consistently observed at appox 35kDa. This band was successfully blocked by incubation with the immunising peptide, and is consistent with the B chain of Cathepsin B-mediated cleavage product of PLAU (Kobayashi et al, J Biol Chem. 1991 Mar 15;266(8):5147-52; PMID: 1900515).
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Expressed in the prostate gland and prostate cancers.
Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
Kachgal S & Putnam AJ Mesenchymal stem cells from adipose and bone marrow promote angiogenesis via distinct cytokine and protease expression mechanisms. Angiogenesis14:47-59 (2011).
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Al-Souhibani N et al. The RNA-binding zinc-finger protein tristetraprolin regulates AU-rich mRNAs involved in breast cancer-related processes. Oncogene : (2010).
Read more (PubMed: 20498646) »