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Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples are not required. Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
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Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
|Uric Acid Assay Buffer||WM||1 x 25ml|
|Uric Acid Enzyme Mix||Green||1 vial|
|Uric Acid Probe (in DMSO, anhydrous)||Red||1 x 0.2ml|
|Uric Acid Standard (2 nmol/µl)||Yellow||1 x 1ml|
Our Abpromise guarantee covers the use of ab65344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Uric acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (duplicates; +/- SD).
Uric acid measured in biological fluid (duplicates; +/- SD).