製品の概要

  • 製品名Anti-Uric acid antibody
  • 製品の詳細
    Rabbit polyclonal to Uric acid
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 免疫原

    Synthetic Uric acid conjugated to BSA (Bovine Serum Albumin).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000.
ELISA 1/5000 - 1/10000.
ICC/IF 1/1000 - 1/5000.

ターゲット情報

  • 関連性Uric acid is a major endogenous antioxidant and peroxynitrite scavenger. Xanthine oxidase oxidizes oxypurines such as xanthine and hypoxanthine to uric acid. In humans and higher primates, uric acid is the final oxidation product of purine catabolism. In most other mammals, the enzyme uricase further oxidizes uric acid to allantoin. The loss of uricase in higher primates parallels the similar loss of the ability to synthesize ascorbic acid. This may be because in higher primates uric acid (urate) partially replaces ascorbic acid. Both urate and ascorbate are strong reducing substances (electron donors) and potent antioxidants. In humans, about half the antioxidant capacity of plasma comes from uric acid.

Anti-Uric acid antibody 画像

  • Lane 1 :
    Lane 2 : Anti-Uric acid antibody (ab53000) at 1/1000 dilution

    Lane 1 : Marker
    Lane 2 : Human LNCaP whole cell lysate at 60 µg

    Secondary
    Lane 2 : IRDye 800CW conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution


    Performed under reducing conditions.

    Exposure time : 4 minutes

    This image is courtesy of an Abreview submitted by Francesco Elia Marino

    See Abreview

Anti-Uric acid antibody (ab53000) 使用論文

This product has been referenced in:
  • van de Hoef DL  et al. Plasmodium falciparum-derived uric acid precipitates induce maturation of dendritic cells. PLoS One 8:e55584 (2013). ICC/IF . Read more (PubMed: 23405174) »
  • Guerreiro S  et al. Protection of midbrain dopaminergic neurons by the end-product of purine metabolism uric acid: potentiation by low-level depolarization. J Neurochem 109:1118-28 (2009). ICC/IF . Read more (PubMed: 19302482) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Human Cell lysate - whole cell (LNCaP cell line)
Specification LNCaP cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 May 15 2014

Thank you very much for contacting us with your enquiry.
To our knowledge, ab53000 has not been tested in IHC-P. I can offer a free primary antibody on a future purchase if you'd like to purchase ab53000 now, test it in IHC-P, and submit feedback ...

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Thank you for contacting Abcam regarding ab53000.


We have not specifically tested this antibody on tissue sections, so we do not have any information about how to use the antibody in this context.


In terms of a positive cont...

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I am very pleased to hear you would like to accept our offer and test ab53000 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"