製品の概要

  • 製品名Anti-UQCRQ antibody [1H9DE5DG5BC8]
    UQCRQ 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1H9DE5DG5BC8] to UQCRQ
  • アプリケーション適用あり: Flow Cyt, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Bovine Complex III subunit 8 (UQCRQ)

  • ポジティブ・コントロール
    • Human, Cow, Rat or Mouse heart mitochondrial lysate
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/1. Predicted molecular weight: 6 kDa.

ターゲット情報

  • 機能This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.
  • 関連疾患Defects in UQCRQ are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.
  • 配列類似性Belongs to the UQCRQ/QCR8 family.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Complex III subunit 8 antibody
    • Complex III subunit VIII antibody
    • Cytochrome b-c1 complex subunit 8 antibody
    • QCR8 antibody
    • QCR8_HUMAN antibody
    • QP-C antibody
    • QPC antibody
    • Ubiquinol-cytochrome c reductase complex 9.5 kDa protein antibody
    • Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C antibody
    • Uqcrq antibody
    see all

Anti-UQCRQ antibody [1H9DE5DG5BC8] 画像

  • Overlay histogram showing HepG2 cells stained with ab110255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110255, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-UQCRQ antibody [1H9DE5DG5BC8] (ab110255) at 1 µg/ml

    Lane 1 : Human mitochondrial lysate at 10 µg
    Lane 2 : Cow mitochondrial lysate at 1 µg
    Lane 3 : Rat mitochondrial lysate at 10 µg
    Lane 4 : Mouse mitochondrial lysate at 10 µg


    Predicted band size : 6 kDa

Anti-UQCRQ antibody [1H9DE5DG5BC8] (ab110255) 使用論文

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Mineri R  et al. How do human cells react to the absence of mitochondrial DNA? PLoS One 4:e5713 (2009). Read more (PubMed: 19492094) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab110255.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"