製品の概要

  • 製品名Anti-UQCRFS1 antibody [5A5]
    UQCRFS1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5A5] to UQCRFS1
  • アプリケーション適用あり: WB, ICC, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Bovine Complex III

  • ポジティブ・コントロール
    • Human, bovine, rat and murine heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in Raji cells.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa.
ICC Use a concentration of 5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
    The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.
  • 配列類似性Contains 1 Rieske domain.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Complex III subunit 5 antibody
    • Complex III subunit IX antibody
    • Cytochrome b c1 complex subunit Rieske mitochondrial antibody
    • Cytochrome b-c1 complex subunit 11 antibody
    • Cytochrome b-c1 complex subunit 5 antibody
    • Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
    • petC antibody
    • PGR1 antibody
    • Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
    • Proton gradient regulation protein 1 antibody
    • Rieske iron sulfur protein antibody
    • Rieske iron-sulfur protein antibody
    • RIP1 antibody
    • RIS1 antibody
    • RISP antibody
    • Ubiquinol cytochrome C reductase rieske iron sulphur antibody
    • Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
    • Ubiquinol-cytochrome c reductase 8 kDa protein antibody
    • Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
    • UCRI_HUMAN antibody
    • UQCR5 antibody
    • UQCRFS1 antibody
    see all

Anti-UQCRFS1 antibody [5A5] 画像

  • All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 0.5 µg/ml

    Lane 1 : Hela Whole Cell Lysate - Hydroxyurea Treated
    Lane 2 : Hela Whole Cell Lysate - Bleomycin Treated
    Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 1 µg/ml

    Lane 1 : Isolated mitochondria from human heart at 5 µg
    Lane 2 : Isolated mitochondria from bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg
  • ab24746 staining UQCRFS1 in murine cardiac myocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in 4% paraformaldehyde and permeabilized using 0.1% Triton X100. Samples were blocked using 10% horse serum for 1 hour at room temperature then incubated with ab14746 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor488 conjugated goat anti-mouse IgG, used at a 1/500 dilution.

    See Abreview

  • Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-UQCRFS1 antibody [5A5] (ab14746) 使用論文

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Seiferling D  et al. Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep 17:953-64 (2016). Read more (PubMed: 27154400) »

See all 22 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (flight muscles)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 40 µg
Specification flight muscles
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (h9c2 cell)
Loading amount 30 µg
Specification h9c2 cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Mar 12 2013


ab14746 can be used in a western bot under denaturing conditions i.e using SDS. Indeed our customers have found that this antibody gives good results under reducing and denaturing conditions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8.0
Username

Ms. Philippa Hepplewhite

Verified customer

投稿 Aug 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Gastric mucosa)
Loading amount 20 µg
Specification Gastric mucosa
Gel Running Conditions Reduced Denaturing (8-16%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Maria Paola Simula

Verified customer

投稿 Jun 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"