Anti-UQCRC2 抗体 [13G12AF12BB11] (ab14745)

製品の概要

  • 製品名
    Anti-UQCRC2 antibody [13G12AF12BB11]
    UQCRC2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [13G12AF12BB11] to UQCRC2
  • アプリケーション
    適用あり: Flow Cyt, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Tissue, cells or virus corresponding to Cow UQCRC2.

  • ポジティブ・コントロール
    • Human, bovine, murine and rat heart mitochondria. HepG2 cell lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IF Use a concentration of 0.1 - 0.5 µg/ml.

Requires heat-induced antigen retrieval where aldehydes are used as fixatives.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/1500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49.5 kDa.

ターゲット情報

  • 機能
    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • 配列類似性
    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Anti-UQCRC2 antibody [13G12AF12BB11] 画像

  • Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 1/1000 dilution + H23 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of ab14745 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14745, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab14745 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14745, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

  • All lanes : Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 0.5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) 使用論文

This product has been referenced in:
  • Gómez-Serrano M  et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . Read more (PubMed: 28064117) »
  • Boominathan A  et al. Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant. Nucleic Acids Res N/A:N/A (2016). Read more (PubMed: 27596602) »

See all 37 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18%)
Loading amount
25 µg
Specification
fibroblasts, mitochondrial fraction
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 12 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
AML12 hepatocytes, mitochondrial fraction
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

投稿 Aug 22 2017

Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - other (whole fly mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
whole fly mitochondrial fraction
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

投稿 Aug 21 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% GEL)
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Specification
Mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

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投稿 May 22 2014

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Human Cultured Cells (H460)
Specification
H460
Permeabilization
Yes - 0.5% Triton-X
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 May 20 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% GEL)
Sample
Human Cell lysate - whole cell (H23)
Specification
H23
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 17 2014

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

I hope this information is helpful...

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Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:


ab14713
ab110244
ab14715
ab14745
ab14705

These have been tested in blue nat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brown Adipose Tissue)
Specification
Brown Adipose Tissue
Fixative
Paraformaldehyde
Permeabilization
No
Username

Ms. Philippa Hepplewhite

Verified customer

投稿 Aug 03 2012

Thank you for your call earlier.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.
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