製品の概要

  • 製品名Anti-UPF3B/RENT3B antibody
    UPF3B/RENT3B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UPF3B/RENT3B
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: MKEEKEHRPK EKRVTLLTPA GATGSGGGTS GDSSKGEDKQ DRNKEKKEAL, corresponding to N terminal amino acids 1-50 of Human UPF3B/RENT3B

  • ポジティブ・コントロール
    • Jurkat cells. Stomach.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

ターゲット情報

  • 関連性UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF3B binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X (referenced from entrez gene).
  • 細胞内局在Nucleus. Cytoplasm. Note: Shuttling between the nucleus and the cytoplasm.
  • 参照データベース
  • 別名
    • HUPF3B antibody
    • hUpf3p X antibody
    • MRXS14 antibody
    • Nonsense mRNA reducing factor 3B antibody
    • Regulator of nonsense transcripts 3B antibody
    • RENT3B antibody
    • Up-frameshift suppressor 3 homolog B antibody
    • Up-frameshift suppressor 3 homolog on chromosome X antibody
    • UPF3 regulator of nonsense transcripts homolog B (yeast) antibody
    • UPF3 regulator of nonsense transcripts homolog B antibody
    • UPF3B antibody
    • UPF3X antibody
    see all

Anti-UPF3B/RENT3B antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling UPF3B/RENT3B with ab66753 at 4-8µg/ml. Arrows indicate positively labeling epithelial cells of the fundic gland. Magnification: 400X.

  • Anti-UPF3B/RENT3B antibody (ab66753) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Anti-UPF3B/RENT3B antibody (ab66753) 使用論文

ab66753 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"