製品の概要

  • 製品名Anti-uPA Receptor antibody
    uPA Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to uPA Receptor
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    KSGCNHPDLDVQYRS

    corresponding to C terminal amino acids 290-304 of Human uPA Receptor, identical to the related Mouse and Rat sequence.

  • ポジティブ・コントロール
    • Human placenta, Rat brain, Rat thymus and Rat heart tissue lysates; MCF7, SMMC, HeLa, Raji and Colo320 whole cell lysates; MCF7 cells

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • 組織特異性Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • 配列類似性Contains 3 UPAR/Ly6 domains.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 87 antibody
    • CD87 antibody
    • CD87 antigen antibody
    • MO 3 antibody
    • MO3 antibody
    • Monocyte activation antigen Mo3 antibody
    • Plasminogen activator receptor urokinase antibody
    • Plasminogen activator urokinase receptor antibody
    • PLAUR antibody
    • U PAR antibody
    • u plasminogen activator receptor antibody
    • U-PAR antibody
    • u-plasminogen activator receptor form 2 antibody
    • UPA receptor antibody
    • uPAR antibody
    • UPAR_HUMAN antibody
    • Urinary plasminogen activator receptor antibody
    • URKR antibody
    • Urokinase plasminogen activator receptor antibody
    • Urokinase plasminogen activator surface receptor antibody
    • urokinase-type plasminogen activator (uPA) receptor antibody
    see all

Anti-uPA Receptor antibody 画像

  • All lanes : Anti-uPA Receptor antibody (ab103791) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat thymus tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : MCF7 whole cell lysate
    Lane 6 : SMMC whole cell lysate
    Lane 7 : HeLa whole cell lysate
    Lane 8 : Raji whole cell lysate
    Lane 9 : Colo320 whole cell lysate


    Predicted band size : 37 kDa
  • ab103791, at 1µg/ml, staining uPA Receptor in MCF7 cells by Immunocytochemistry.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa

    MDA-MB-231 cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor in MDA-MB-231 cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

Anti-uPA Receptor antibody (ab103791) 使用論文

This product has been referenced in:
  • Al Dhaheri Y  et al. Anti-metastatic and anti-tumor growth effects of Origanum majorana on highly metastatic human breast cancer cells: inhibition of NF?B signaling and reduction of nitric oxide production. PLoS One 8:e68808 (2013). WB . Read more (PubMed: 23874773) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9
Sample Mouse Tissue sections (Lymph Nodes)
Specification Lymph Nodes
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Apr 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample Human Tissue sections (Placenta)
Specification Placenta
Permeabilization Yes - Wash buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Apr 01 2015

Thank you for your inquiry.

I heard back from the testing laboratory that the 70kDa is the molecular weight of glycosylated uPAR.

I hope this information helps. Please contact us with any other questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lungs)
Loading amount 40 µg
Specification Lungs
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

投稿 Jul 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"