Rabbit polyclonal to UNK unkempt
Mouse, Cow, Dog
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human UNK unkempt.
(Peptide available as
This antibody gave a positive signal in HeLa Nuclear Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the unkempt family.
Contains 5 C3H1-type zinc fingers. Contains 1 RING-type zinc finger.
Information by UniProt
RING finger protein unkempt homolog antibody
Western blot - Anti-UNK unkempt antibody (ab78391)
Anti-UNK unkempt antibody (ab78391) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 88 kDa Observed band size: 88 kDa Additional bands at: 35 kDa, 48 kDa. We are unsure as to the identity of these extra bands. Exposure time: 3 minutes
Immunocytochemistry/ Immunofluorescence - Anti-UNK unkempt antibody (ab78391)
ICC/IF image of ab78391 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78391, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
has not yet been referenced specifically in any publications.
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