製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to UNG
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UNG.

    (Peptide available as ab25856.)

  • ポジティブ・コントロール
    • In WB, this antibody gave a positive signal in HeLa and Jurkat whole cell lysates, and in HeLa nuclear lysate. In IF, we observed positive staining in HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
IHC-P
  • 追加情報
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35, 39 kDa representing two alternatively spliced forms of UNG that are differentially localized (predicted molecular weight: 33, 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
    • 組織特異性
      Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
    • 関連疾患
      Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
    • 配列類似性
      Belongs to the uracil-DNA glycosylase family.
    • 翻訳後修飾
      Isoform 1 is processed by cleavage of a transit peptide.
    • 細胞内局在
      Mitochondrion and Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DGU antibody
      • DKFZp781L1143 antibody
      • HIGM 4 antibody
      • HIGM4 antibody
      • OTTHUMP00000240527 antibody
      • OTTHUMP00000240528 antibody
      • OTTHUMP00000240529 antibody
      • UDG antibody
      • UNG 1 antibody
      • UNG 15 antibody
      • ung antibody
      • UNG_HUMAN antibody
      • UNG1 antibody
      • UNG15 antibody
      • UNG2 antibody
      • Uracil DNA glycosylase 1 antibody
      • Uracil DNA glycosylase 2 antibody
      • Uracil DNA glycosylase antibody
      • Uracil-DNA glycosylase antibody
      see all

    画像

    • All lanes : Anti-UNG antibody (ab23926) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : HeLa (Human epithelial carcinoma cell line) nuclear lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Predicted band size: 33, 35 kDa
      Observed band size: 35,39 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 18 kDa (possible cleavage fragment), 18 kDa (possible cross reactivity), 23 kDa (possible cleavage fragment), 23 kDa (possible cross reactivity), 40 kDa (possible cross reactivity)



      UNG is present in the cell in two isoforms, that differ at their amino-terminal ~40 amino acids. This results in one isoform that is present in the nucleus and one that is present in the mitochondria. This antibody strongly recognizes a doublet at approximately 35 and 39 kDa which closely correspond in size to these two isoforms (whose predicted molecular weights are 33 and 35 kDa).
    • ICC/IF image of ab23926stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23926, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • Image courtesy of Human Protein Atlas

      ab23926 staining UNG in human heart muscle. Paraffin embedded human heart muscle tissue was incubated with ab23926 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab23926 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

      Further results for this antibody can be found at www.proteinatlas.org

    参考文献

    This product has been referenced in:
    • Su MT  et al. Uracil DNA glycosylase BKRF3 contributes to Epstein-Barr virus DNA replication through physical interactions with proteins in viral DNA replication complex. J Virol 88:8883-99 (2014). Read more (PubMed: 24872582) »
    • Kitamura K  et al. Uracil DNA glycosylase counteracts APOBEC3G-induced hypermutation of hepatitis B viral genomes: excision repair of covalently closed circular DNA. PLoS Pathog 9:e1003361 (2013). WB ; Human . Read more (PubMed: 23696735) »

    See all 7 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (CH12 (B cell lymphoma))
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    CH12 (B cell lymphoma)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 24 2017

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (MCF-7 breast cancer cells)
    Negative control
    See figure
    Specification
    MCF-7 breast cancer cells
    Detection step
    Semiquantitative PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    See figure
    Username

    Abcam user community

    Verified customer

    投稿 May 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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