製品の概要

  • 製品名
    Anti-UNC5D antibody
  • 製品の詳細
    Mouse monoclonal to UNC5D
  • アプリケーション
    適用あり: WB, ICC/IF, Flow Cyt, IHC - Wholemountmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
  • 免疫原

    Recombinant fragment: TDNGEALPES IPSAPGTLPH FIEEPDDAYI IKSNPIALRC KARPAMQIFF KCNGEWVHQN EHVSEETLDE SSGLKVREVF INVTRQQVED FHGPEDYWCQ , corresponding to amino acids 31-131 of Human UNC5D

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC - Wholemount Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    UNC5D belongs to a family of netrin 1 receptors thought to mediate the chemorepulsive effect of netrin 1 on specific axons. On ligand binding UNC5D may mediate axon repulsion of neuronal growth cones in the developing nervous system.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • FLJ16019 antibody
    • KIAA1777 antibody
    • Netrin receptor UNC5D antibody
    • Netrin receptor Unc5h4 antibody
    • PRO34692 antibody
    • Protein unc 5 homolog D antibody
    • Unc 5 homolog 4 antibody
    • Unc 5 homolog D (C. elegans) antibody
    • Unc 5 homolog D antibody
    • UNC 5D antibody
    • Unc5h4 antibody
    see all

画像

  • Overlay histogram showing SH-SY5Y cells stained with ab58141 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58141, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Western blot against tagged recombinant protein immunogen using ab58141 UNC5D antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab58141 staining UNC5D in chicken whole mount brain by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 10% serum for 48 hours. Samples were then incubated with primary antibody at 1/500 for 48 hours. The secondary antibody used was a goat anti-mouse monoclonal conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

参考文献

This product has been referenced in:

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate buffer, pH 6
Sample
Mouse Tissue sections (E17 mouse brain neocortex)
Specification
E17 mouse brain neocortex
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Bhavin Shah

Verified customer

投稿 Jul 17 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (Embryonic brain (cortex), E15.5)
Specification
Embryonic brain (cortex), E15.5
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 11 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (Mouse Cortical neurons, E14.5)
Specification
Mouse Cortical neurons, E14.5
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 11 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Antigen retrieval step
None
Sample
Ferret Tissue sections (Forebrain)
Specification
Forebrain
Permeabilization
Yes - 0.3% Triton-X
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 06 2013

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I am very pleased to hear you would like to accept our offer and test ab58141in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem t...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (whole-mount brain)
Specification
whole-mount brain
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Oct 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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