製品の概要

  • 製品名Anti-UNC5C antibody
    UNC5C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UNC5C
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the center region of Human UNC5C conjugated to KLH (NP_003719.2)

  • ポジティブ・コントロール
    • K562 cell lysate# This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab91408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 103 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
    • 組織特異性Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.
    • 配列類似性Belongs to the unc-5 family.
      Contains 1 death domain.
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 2 TSP type-1 domains.
      Contains 1 ZU5 domain.
    • 翻訳後修飾Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent.
      Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • homolog of C. elegans transmembrane receptor Unc5 antibody
      • Netrin receptor UNC5C antibody
      • Protein unc 5 homolog C antibody
      • Protein unc-5 homolog 3 antibody
      • Protein unc-5 homolog C antibody
      • Unc 5 homolog 3 antibody
      • Unc 5 homolog C antibody
      • Unc5 (C.elegans homolog) c antibody
      • Unc5c antibody
      • UNC5C_HUMAN antibody
      • UNC5H3 antibody
      see all

    Anti-UNC5C antibody 画像

    • ICC/IF image of ab91408 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91408 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-UNC5C antibody (ab91408) at 1/100 dilution + K562 cell lysate at 35 µg

      Predicted band size : 103 kDa

    Anti-UNC5C antibody (ab91408) 使用論文

    ab91408 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab91408.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"