Anti-Unc5a 抗体 (ab81165)


  • 製品名Anti-Unc5a antibody
    Unc5a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Unc5a
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a section of internal sequence amino acids 323-372 of Unc5a: ILVYCRKKEG LDSDVADSSI LTSGFQPVSI KPSKADNPHL LTIQPDLSTT

  • ポジティブ・コントロール
    • HeLa cell lysate



Our Abpromise guarantee covers the use of ab81165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre in peptide based assay is 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 機能Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
  • 配列類似性Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 TSP type-1 domain.
    Contains 1 ZU5 domain.
  • ドメインThe ZU5 domain mediates the interaction with MAGED1, which participates in the induction of apoptosis.
  • 翻訳後修飾Phosphorylated on cytoplasmic tyrosine residues. Phosphorylated by PKC in vitro.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
  • 細胞内局在Cell membrane. The interaction with PRKCABP regulates its surface expression and leads to its removal from surface of neurons and growth cones.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ16449 antibody
    • KIAA1976 antibody
    • Netrin receptor UNC 5A antibody
    • Netrin receptor UNC5A antibody
    • Netrin receptor Unc5h1 antibody
    • Protein unc-5 homolog 1 antibody
    • Protein unc-5 homolog A antibody
    • Unc 5 homolog 1 antibody
    • Unc 5 homolog A antibody
    • Unc 5a antibody
    • Unc5 a antibody
    • Unc5 homolog 1 antibody
    • Unc5 homolog A antibody
    • Unc5a antibody
    • UNC5A_HUMAN antibody
    • Unc5h1 antibody
    see all

Anti-Unc5a antibody 画像

  • Anti-Unc5a antibody (ab81165) at 1 µg/ml + HeLa cell lysate at 10 µg

    Use at 1/100000

    Predicted band size : 93 kDa

Anti-Unc5a antibody (ab81165) 使用論文

ab81165 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0.25%TritonX100
Fixative Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 13 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Sample African Green Monkey Cell lysate - whole cell (COS cells transfected with human Unc5)
Specification COS cells transfected with human Unc5
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Alain Chedotal

Verified customer

投稿 Nov 04 2014

The observed MW for this antibody is in fact lower than the predicted MW. Generally a lower observed MW is the results form isoforms produced from splice variants. There are in fact 4 predicted isoforms for the UNC5 target and most likely the observed...

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