製品の概要

  • 製品名Anti-umuD antibody
  • 製品の詳細
    Rabbit polyclonal to umuD
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Purified recombinant LacZ-UmuD fusion protein

  • ポジティブ・コントロール
    • Protein extract from E. coli

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/3000. Predicted molecular weight: 14 kDa (processed form. Precursor form is 17 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性UmuD is a DNA polymerase V subunit capable of translesion synthesis and inducible mutagenesis, by radiation or chemical agents. Transcription of umuD is repressed by a repressor, LexA protein in uninduced cells. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as a error-prone translesion DNA polymerase. The molecular weight of the intact UmuD is 17kD and the proteolytically processed active form is 14KD.
    • 参照データベース
      • 別名
        • DNA polymerase V subunit D antibody
        • ECK1171 antibody
        • JW1172 antibody
        • Protein umuD antibody

      Anti-umuD antibody 画像

      • All lanes : Anti-umuD antibody (ab91343) at 1/3000 dilution

        Lane 1 : protein extracts from E. coli DE274 (lexA51, recA730)
        Lane 2 : protein extract from E. coli DE274 (lexA51, recA730) treated with mitomycin C


        Predicted band size : 14 kDa

      Anti-umuD antibody (ab91343) 使用論文

      This product has been referenced in:
      • Naiman K  et al. Chronology in lesion tolerance gives priority to genetic variability. Proc Natl Acad Sci U S A 111:5526-31 (2014). Read more (PubMed: 24706928) »
      • Cafarelli TM  et al. A single residue unique to DinB-like proteins limits formation of the polymerase IV multiprotein complex in Escherichia coli. J Bacteriol 195:1179-93 (2013). Escherichia coli . Read more (PubMed: 23292773) »

      See all 2 Publications for this product

      Product Wall

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Western blot
      Sample Escherichia coli Cell lysate - whole cell (bacteria)
      Loading amount 1e+009 cells
      Specification bacteria
      Gel Running Conditions Non-reduced Denaturing (15%)
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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      Verified customer

      投稿 Feb 12 2013

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"