製品の概要

  • 製品名Anti-ULP-1 antibody
  • 製品の詳細
    Rabbit polyclonal to ULP-1
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Yeast ULP 1. It does not react with ULP 1 from related sources including Human SENP.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant Yeast ULP 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 72.4 kDa (predicted molecular weight: 80 kDa).
ELISA 1/4000 - 1/20000.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性ULP1, ubiquitin-like protein-specific protease 1, initially processes Smt3 and also acts as a deconjugating enzyme for Smt3 [Saccharomyces cerevisiae (Baker's yeast)]. Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO 1 and the close homologues SUMO 2 and SUMO 3. Three distinct steps can be distinguished in the SUMO modification pathway: 1) activation of SUMO, 2) transfer of SUMO to the conjugating enzyme, and 3) substrate modification. Since SUMO is synthesized as a precursor protein, a maturation step precedes the activation reaction. In yeast, C terminal processing of the SUMO precursor is mediated by the processing protease ULP 1 which has an additional role in the deconjugation of SUMO-modified substrates. Mature SUMO is activated by SUMO-activating enzyme, an E1-like heterodimeric protein complex composed of Uba2 and Aos1. ULP 1 function has provided evidence that SUMO modification in yeast, as has been suspected for vertebrates, plays an important role in nucleocytoplasmic trafficking.
  • 細胞内局在Nuclear
  • 別名
    • Probable sentrin specific protease antibody
    • Ubiquitin Like Protease antibody

Anti-ULP-1 antibody 画像

  • Anti-ULP-1 antibody (ab34668) at 1/1000 dilution + Truncated ULP 1 fusion protein (arrow-head) with 5% non-fat dry milk in TTBS

    Secondary
    1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.

    Predicted band size : 80 kDa

Anti-ULP-1 antibody (ab34668) 使用論文

ab34668 has not yet been referenced specifically in any publications.

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Thank you very much for your inquiry.

It is very likely that the ab34668 will recognize the fragment of amino acid 403 to 600 of ULP-1.

The antibody ab34668 has been raised against the wholerecombinant protein, and should therefore ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"