製品の概要

  • 製品名Anti-ULK3 antibody [EPR4888]
    ULK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4888] to ULK3
  • アプリケーション適用あり: WB, ICC, Flow Cyt, IHC-Pmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of Human ULK3.

  • ポジティブ・コントロール
    • IHC-P: Human bladder carcinoma tissue WB: Human heart, testis, fetal kidney, LnCaP and 293T cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR4888
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
ICC 1/25 - 1/50.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
    • 組織特異性Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
    • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 2 MIT domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated in vitro.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 製品の状態There are 3 isoforms produced by alternative splicing.
    • 別名
      • DKFZP434C131 antibody
      • FLJ90566 antibody
      • Serine/threonine-protein kinase ULK3 antibody
      • Ulk3 antibody
      • ULK3_HUMAN antibody
      • unc 51 like kinase 3 (C. elegans) antibody
      • Unc-51-like kinase 3 antibody
      see all

    Anti-ULK3 antibody [EPR4888] 画像

    • All lanes : Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Human testis lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : LnCaP cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124947, at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    Anti-ULK3 antibody [EPR4888] (ab124947) 使用論文

    This product has been referenced in:
    • Hutterer C  et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013). WB ; Human . Read more (PubMed: 23648710) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"