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RabMAb

Anti-ULK3 抗体 [EPR4888] (ab124947)

製品の概要

  • 製品名
    Anti-ULK3 antibody [EPR4888]
    ULK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4888] to ULK3
  • アプリケーション
    適用あり: WB, ICC, Flow Cyt, IHC-Pmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of Human ULK3.

  • ポジティブ・コントロール
    • IHC-P: Human bladder carcinoma tissue WB: Human heart, testis, fetal kidney, LnCaP and 293T cell lysates
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR4888
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
ICC 1/25 - 1/50.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
    • 組織特異性
      Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
    • 配列類似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 2 MIT domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Autophosphorylated in vitro.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 製品の状態
      There are 3 isoforms produced by alternative splicing.
    • 別名
      • DKFZP434C131 antibody
      • FLJ90566 antibody
      • Serine/threonine-protein kinase ULK3 antibody
      • Ulk3 antibody
      • ULK3_HUMAN antibody
      • unc 51 like kinase 3 (C. elegans) antibody
      • Unc-51-like kinase 3 antibody
      see all

    Anti-ULK3 antibody [EPR4888] 画像



    • Predicted band size : 53 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ULK3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Hek293 whole cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab124947 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124947 was shown to specifically react with ULK3 when ULK3 knockout samples were used. Wild-type and ULK3 knockout samples were subjected to SDS-PAGE. Ab124947 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Human testis lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : LnCaP cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124947, at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    Anti-ULK3 antibody [EPR4888] (ab124947) 使用論文

    This product has been referenced in:
    • Hutterer C  et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013). WB ; Human . Read more (PubMed: 23648710) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab124947.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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