製品の概要

  • 製品名
    Anti-ULBP3 antibody [MM0594-6E12]
  • 製品の詳細
    Mouse monoclonal [MM0594-6E12] to ULBP3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human ULBP3 protein

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
  • ポリ/モノ
    モノクローナル
  • クローン名
    MM0594-6E12
  • アイソタイプ
    IgG2
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    ULBP3 is a human ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. ULBP3 has a lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1.
  • 細胞内局在
    Cell Membrane
  • 参照データベース
  • 別名
    • ALCAN- gamma antibody
    • N2DL-3 antibody
    • N2DL3 antibody
    • NKG2D ligand 3 antibody
    • NKG2D ligand 3 precursor antibody
    • NKG2DL3 antibody
    • OTTHUMP00000017411 antibody
    • RAET1N antibody
    • Retinoic acid early transcript 1N antibody
    • UL16 binding protein 3 antibody
    • UL16-binding protein 3 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling ULBP3 with ab89931. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

参考文献

This product has been referenced in:
  • Cho H  et al. MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer 14:957 (2014). IHC-P ; Human . Read more (PubMed: 25510288) »

See 1 Publication for this product

レビューと Q&A

We used the recombinant protein (immunogen) to test the WB and we have the band at 25-30 kDa. I believe you were looking for a WB to test for the endogenous protein but unfortunately there is no WB data using a cell or tissue lysate. Plea...

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Thank you for your enquiry. I have submitted a request for a western blot using ab89931. Unfortunately I was informed that this information could not be provided until Monday. I apologize for the delay and will forward you any data I receive as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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