製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to ULBP2
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human ULBP2 protein (AAH34689).

  • ポジティブ・コントロール
    • Human ovary cancer tissue lysate. Cell lysate from transfected 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
  • 組織特異性
    Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues.
  • 配列類似性
    Belongs to the MHC class I family.
  • 細胞内局在
    Cell membrane. Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALCAN alpha antibody
    • ALCAN-alpha antibody
    • N2DL 2 antibody
    • N2DL-2 antibody
    • N2DL2 antibody
    • N2DL2_HUMAN antibody
    • NKG2D ligand 2 antibody
    • NKG2D ligand 2 precursor antibody
    • NKG2DL2 antibody
    • RAET1H antibody
    • Retinoic acid early transcript 1 H antibody
    • Retinoic acid early transcript 1H antibody
    • UL16 binding protein 2 antibody
    • UL16-binding protein 2 antibody
    • ULBP2 antibody
    see all

画像

  • ab88645 detecting ULBP2 in ULBP2-expressing 293 cells (Green) compared with negative control 293 cells (Black) by flow cytometry.
  • Anti-ULBP2 antibody (ab88645) at 1 µg/ml + Human ovary cancer tissue lysate at 50 µg

    Predicted band size : 27 kDa
    Observed band size : ~35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ULBP2 antibody (ab88645) at 1 µg/ml

    Lane 1 : cell lysate from ULBP2 transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 27 kDa
    Observed band size : ~29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~27 kDa,~33 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Dudek-Peric AM  et al. Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin. Cancer Res 75:1603-14 (2015). WB ; Human . Read more (PubMed: 25762540) »

See 1 Publication for this product

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