製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to UGT2B15
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein corresponding to amino acids 1-530 of Human UGT2B15 (AAI46571.1).

  • ポジティブ・コントロール
    • Human liver lysate, A-431 lysate, UGT2B15 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.

ターゲット情報

  • 機能
    UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens.
  • 組織特異性
    Expressed in many tissues. Present in liver, prostate and testis.
  • 配列類似性
    Belongs to the UDP-glycosyltransferase family.
  • 細胞内局在
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.4.1.17 antibody
    • HLUG4 antibody
    • UDB15_HUMAN antibody
    • UDP glucuronosyltransferase 2 family polypeptide B15 antibody
    • UDP glucuronosyltransferase 2 family, member B15 antibody
    • UDP glucuronosyltransferase 2 family, member B8 antibody
    • UDP glucuronosyltransferase 2B15 antibody
    • UDP glucuronosyltransferase UGT2B15 antibody
    • UDP glucuronyltransferase family 2 beta 15 antibody
    • UDP glycosyltransferase 2 family, member B15 antibody
    • UDP glycosyltransferase 2B15 antibody
    • UDP-glucuronosyltransferase 2B15 antibody
    • UDP-glucuronosyltransferase 2B8 antibody
    • UDPGT 2B15 antibody
    • UDPGT 2B8 antibody
    • UDPGT2B15 antibody
    • UDPGTh 3 antibody
    • UDPGTh-3 antibody
    • UDPGTH3 antibody
    • UGT2B15 antibody
    • UGT2B8 antibody
    • Uridine diphosphate glucuronosyltransferase 2 family, member B8 antibody
    • Uridine diphosphate glycosyltransferase 2 family, member B15 antibody
    see all

画像

  • Anti-UGT2B15 antibody (ab89274) at 1 µg/ml + Human liver lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Anti-UGT2B15 antibody (ab89274) at 1 µg/ml + A-431 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-UGT2B15 antibody (ab89274) at 1 µg/ml

    Lane 1 : UGT2B15 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

プロトコール

参考文献

ab89274 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (LNCaP Androgen Dependent Prostate Cancer Cells)
Loading amount
20 µg
Specification
LNCaP Androgen Dependent Prostate Cancer Cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Skyla Carney

Verified customer

投稿 Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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