製品の概要

  • 製品名
    Anti-UGT1A3 antibody
  • 製品の詳細
    Mouse monoclonal to UGT1A3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: KVLVVPIDGS HWLSMREVLR ELHARGHQAV VLTPEVNMHI KEENFFTLTT YAISWTQDEF DRHVLGHTQL YFETEHFLKK FFRSMAMLNN MSLVYHRSCV , corresponding to amino acids 30-130 of Human UGT1A3

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      The UGT enzymes comprise a superfamily of integral membrane proteins of the endoplasmic reticulum that have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
    • 細胞内局在
      Microsome. Endoplasmic reticulum; Single-pass membrane protein.
    • 参照データベース
    • 別名
      • EC 2.4.1.17 antibody
      • GNT 1 antibody
      • GNT1 antibody
      • UDP glucuronosyltransferase 1 3 antibody
      • UDP glucuronosyltransferase 1 family polypeptide A3 antibody
      • UDP glucuronosyltransferase 1A3 antibody
      • UDP glucuronosyltransferase antibody
      • UDP glycosyltransferase 1 family polypeptide A3 antibody
      • UDPGT antibody
      • UGT 1 antibody
      • UGT 1C antibody
      • UGT1 antibody
      • UGT1 03 antibody
      • UGT1*3 antibody
      • UGT1.3 antibody
      • UGT1C antibody
      see all

    Anti-UGT1A3 antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab57400 UGT1A3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    プロトコール

    Anti-UGT1A3 antibody (ab57400) 使用論文

    This product has been referenced in:
    • Collier AC  et al. UDP-glucuronosyltransferase 1a enzymes are present and active in the mouse blastocyst. Drug Metab Dispos 42:1921-5 (2014). Read more (PubMed: 25200869) »
    • Jarrar YB  et al. Determination of major UDP-glucuronosyltransferase enzymes and their genotypes responsible for 20-HETE glucuronidation. J Lipid Res 55:2334-42 (2014). Read more (PubMed: 25249502) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Ovary)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1.5%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Dec 02 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (Membranes from insect cells)
    Loading amount
    60000 µg
    Specification
    Membranes from insect cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Moshe Finel

    Verified customer

    投稿 Oct 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録