製品の概要

  • 製品名Anti-UGT1A10 antibody
    UGT1A10 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to UGT1A10
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 187-290 of Human UGT1A10 with tag (NP_061948).

  • ポジティブ・コントロール
    • Recombinant fragment of human UGT1A10.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 関連性The UGT enzymes comprise a superfamily of integral membrane proteins of the endoplasmic reticulum that have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
  • 細胞内局在Microsome
  • 参照データベース
  • 別名
    • GNT1 antibody
    • UDP glucuronosyltransferase 1 10 antibody
    • UDP glucuronosyltransferase 1 family polypeptide A10 antibody
    • UDP glucuronosyltransferase 1 J antibody
    • UDP glucuronosyltransferase 1A10 antibody
    • UDP glycosyltransferase antibody
    • UDPGT antibody
    • UDPGT 1 10 antibody
    • UGT 1J antibody
    • UGT1 antibody
    • UGT1 10 antibody
    • UGT1.10 antibody
    • UGT1J antibody
    see all

Anti-UGT1A10 antibody 画像

  • Anti-UGT1A10 antibody (ab76677) at 5 µg/ml + Recombinant fragment of human UGT1A10 at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-UGT1A10 antibody (ab76677) 使用論文

ab76677 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (breast adenocarcinoma, colorectal adenocarcinoma)
Gel Running Conditions Reduced Denaturing (10% resolving, 5% stacking gel)
Loading amount 40 µg
Specification breast adenocarcinoma, colorectal adenocarcinoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Joanna Polewska

Verified customer

投稿 Dec 09 2016

Application Western blot
Sample Human Recombinant protein (Membranes from baculovirus-infected insect cells)
Loading amount 50000 µg
Specification Membranes from baculovirus-infected insect cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Moshe Finel

Verified customer

投稿 Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"