製品の概要

  • 製品名Anti-UGT1A1 antibody
    UGT1A1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UGT1A1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    A 14-22 amino acid sequence within synthetic peptide: VWLFRSDFVK DYPRPIMPNM VFVGGINCLH QNPLSQEFEA YINASGEHGI, corresponding to internal sequence amino acids 253-302 of Human UGT1A1

  • ポジティブ・コントロール
    • Fetal liver cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • Information by UniProt
  • 参照データベース
  • 別名
    • BILIQTL1 antibody
    • Bilirubin specific UDPGT isozyme 1 antibody
    • bilirubin UDP glucuronosyltransferase 1 1 antibody
    • bilirubin UDP glucuronosyltransferase isozyme 1 antibody
    • Bilirubin-specific UDPGT isozyme 1 antibody
    • EC 2.4.1.17 antibody
    • GNT1 antibody
    • HUG BR1 antibody
    • HUG-BR1 antibody
    • HUGBR1 antibody
    • PHENOL/BILIRUBIN UDP GLUCURONOSYLTRANSFERASE antibody
    • UD11_HUMAN antibody
    • UDP glucuronosyltransferase 1 1 [Precursor] antibody
    • UDP glucuronosyltransferase 1 family polypeptide A1 antibody
    • UDP glucuronosyltransferase 1A1 antibody
    • UDP GLYCOSYLTRANSFERASE 1 antibody
    • UDP-glucuronosyltransferase 1-1 antibody
    • UDP-glucuronosyltransferase 1-A antibody
    • UDP-glucuronosyltransferase 1A1 antibody
    • UDPGT antibody
    • UDPGT 1-1 antibody
    • UGT 1A antibody
    • UGT-1A antibody
    • UGT1 antibody
    • UGT1 01 antibody
    • UGT1*1 antibody
    • UGT1-01 antibody
    • UGT1.1 antibody
    • UGT1A antibody
    • Ugt1a1 antibody
    • URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE, BILIRUBIN/PHENOL antibody
    • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 antibody
    • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 FAMILY, POLYPEPTIDE A1 antibody
    see all

Anti-UGT1A1 antibody 画像

  • Anti-UGT1A1 antibody (ab62600) at 2.5 µg/ml + fetal liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

Anti-UGT1A1 antibody (ab62600) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: w/ or w/o 0.01M Sodium Cytrate pH 6.0
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization Yes - w/ or w/o 0.1% Triton
Fixative 4% PFA + 30% Sucrose
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投稿 Oct 07 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization Yes - w/ or w/o 0.1% triton
Fixative Acetone or 0.1%Acetic Acid/Ethanol or Methanol
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投稿 Oct 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Sodium Cytrate pH 6.0
Sample Rat Tissue sections (Liver)
Specification Liver
Permeabilization Yes - w/ or w/o 0.025% Triton
Fixative Formaldehyde
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投稿 Oct 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (ES-derived hepatocytes)
Specification ES-derived hepatocytes
Permeabilization Yes - BSA / Tween 0.1%
Fixative Methanol
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投稿 Sep 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing (NuPAGE Novex 4-12% Bis-Tris midi gel (Invitrogen))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Xia Wen

Verified customer

投稿 Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"