Anti-UFD1L 抗体 (ab96648)
Key features and details
- Rabbit polyclonal to UFD1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UFD1L antibody
UFD1L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UFD1L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Zebrafish -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 277 of UFD1L (NP_005650; UniProt ID: Q92890 isoform short).
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ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: A431 cell IHC-P: H441 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96648の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. -
組織特異性
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney. -
パスウェイ
Protein degradation; proteasomal ubiquitin-dependent pathway. -
配列類似性
Belongs to the UFD1 family. -
細胞内局在
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 7353 Human
- Entrez Gene: 22230 Mouse
- Entrez Gene: 84478 Rat
- Omim: 601754 Human
- SwissProt: Q92890 Human
- SwissProt: P70362 Mouse
- SwissProt: Q9ES53 Rat
- Unigene: 474213 Human
see all -
別名
- UB fusion protein 1 antibody
- Ubiquitin fusion degradation 1 like (yeast) antibody
- Ubiquitin fusion degradation 1 like antibody
see all
画像
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All lanes : Anti-UFD1L antibody (ab96648) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE -
ab96648, at a 1/200 dilution, staining UFD1L in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe -
ab96648, at a 1/500 dilution, staining UFD1L in paraffin embedded H441 xenograft by Immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab96648 は 2 報の論文で使用されています。
- Wrobel L et al. Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance. Nat Commun 13:4146 (2022). PubMed: 35842429
- Meyer C et al. The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Mol Cell 69:622-635.e6 (2018). PubMed: 29429924