製品の概要

  • 製品名Anti-UCP3 antibody
    UCP3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UCP3
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human Skeletal Muscle and Heart tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal Heart muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

  • 機能UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • 組織特異性Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • 関連疾患Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 配列類似性Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態UCP3 is preferentially expressed in muscle
  • 別名
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

Anti-UCP3 antibody 画像

  • All lanes : Anti-UCP3 antibody (ab125830) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) - (Negative Control)
    Lane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)- (Negative Control)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
    The predicted molecular weight of UCP3 is 34 kDa (SwissProt), however we expect to observe a banding pattern between 45 and 50 kDa. Please note that both Human Liver and Kidney tissue lysates were used as negative controls for UCP3. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125830 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-UCP3 antibody (ab125830) 使用論文

ab125830 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cancer cells)
Specification A549 Lung cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"