Anti-UCP3 抗体 (ab10985)
Key features and details
- Rabbit polyclonal to UCP3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UCP3 antibody
UCP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UCP3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human UCP3 aa 254-267 conjugated to keyhole limpet haemocyanin.
Database link: P55916 -
特記事項
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody is affinity-purified using the immunogen peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10985の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/3000.
|
特記事項 |
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IHC-P
1/3000. |
ターゲット情報
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機能
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. -
組織特異性
Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles. -
関連疾患
Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7352 Human
- Omim: 602044 Human
- SwissProt: P55916 Human
- Unigene: 101337 Human
- Unigene: 621879 Human
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製品の状態
UCP3 is preferentially expressed in muscle -
別名
- Mitochondrial uncoupling protein 3 antibody
- SLC25A9 antibody
- Solute carrier family 25 member 9 antibody
see all
画像
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ab10985 (2µg/ml) staining UCP3 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of myocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab10985 は 20 報の論文で使用されています。
- Yeh CC et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep 25:N/A (2022). PubMed: 34913071
- Beleza J et al. Gestational Exercise Increases Male Offspring's Maximal Workload Capacity Early in Life. Int J Mol Sci 23:N/A (2022). PubMed: 35409278
- Chazarin B et al. Molecular Liver Fingerprint Reflects the Seasonal Physiology of the Grey Mouse Lemur (Microcebus murinus) during Winter. Int J Mol Sci 23:N/A (2022). PubMed: 35457071
- Davis MS & Barrett MR Effect of conditioning and physiological hyperthermia on canine skeletal muscle mitochondrial oxygen consumption. J Appl Physiol (1985) 130:1317-1325 (2021). PubMed: 33661725
- Lee K et al. Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice. Cells 9:N/A (2020). PubMed: 32041272