製品の概要

  • 製品名Anti-UCP1 antibody
    UCP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UCP1
  • 特異性ab10983 has been found not to work in Human samples in Western Blotting, but was shown to work in IHC-P and ICC/IF. The antibody does not cross-react with UCP2 or UCP3.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Spermophilus tridecemlineatus
    交差が予測される動物種: Sheep, Rabbit, Cow, Dog, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 145-159 of Human UCP1, with N-terminal cysteine added.

  • ポジティブ・コントロール
    • Brown adipose tissue.
  • 特記事項Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500. PubMed: 22545021
WB 1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
IHC-P 1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.

ターゲット情報

  • 機能UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • 組織特異性Brown adipose tissue.
  • 配列類似性Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態UCP1 is preferentially expressed in brown adipose tissue
  • 別名
    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Anti-UCP1 antibody 画像

  • All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml

    Lane 1 : Rat brown fat
    Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)

    Secondary
    Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
  • ab10983 staining UCP1 in Human brown adipose tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% triton for 20 minutes and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer, pH 6. Samples were incubated with primary antibody (1/250 in PBS + 1% BSA) for 18 hours at 4°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab10983, staining UCP1 (green), in undifferentiated (left) and differentiated (right) murine fat progenitor cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were incubated with primary antibody at 1/500 dilution. An AlexaFluor®546-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained using DAPI (blue).
  • ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin.

Anti-UCP1 antibody (ab10983) 使用論文

This product has been referenced in:
  • Laurila PP  et al. USF1 deficiency activates brown adipose tissue and improves cardiometabolic health. Sci Transl Med 8:323ra13 (2016). WB . Read more (PubMed: 26819196) »
  • Elias I  et al. ALOX5AP Overexpression in Adipose Tissue Leads to LXA4 Production and Protection Against Diet-Induced Obesity and Insulin Resistance. Diabetes 65:2139-50 (2016). Read more (PubMed: 27207555) »

See all 64 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (mesenchymal stem cells)
Permeabilization Yes - Triton 0.25%
Specification mesenchymal stem cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Jul 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (fetal visceral fat)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization No
Specification fetal visceral fat
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative Paraformaldehyde
Username

Mr. Jeremy Gingrich

Verified customer

投稿 Apr 15 2016

Application Western blot
Sample Sheep Cell lysate - whole cell (preadipocyte)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% PAG)
Loading amount 10 µg
Specification preadipocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Jeremy Gingrich

Verified customer

投稿 Apr 15 2016

Application Western blot
Sample Sheep Cell lysate - whole cell (Ovine brown adipocyte precursor cells)
Gel Running Conditions Reduced Denaturing (12.5% gel)
Loading amount 30 µg
Treatment 9 day induction of differentiation with a serum-free Dulbecco's modified Eagle Ham/F12 medium supplemented with 100 nM dexamethasone, 1 μg/mL insulin, 10 pg/mL transferrin, and 1 nM triiodothyronine
Specification Ovine brown adipocyte precursor cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jan 04 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brown adipose tissue)
Permeabilization Yes - 0.1% Triton X-100
Specification Brown adipose tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
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投稿 Oct 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization No
Specification breast tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
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投稿 Aug 17 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mammary gland)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 95C
Permeabilization Yes - PBS with 3% Triton
Specification Mammary gland
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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投稿 Aug 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brown fat)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization No
Specification Brown fat
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% formalin
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投稿 Jul 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (White and brown fat)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6 citrate
Permeabilization No
Specification White and brown fat
Blocking step Dako dual enzyme and protein blocks as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative Formaldehyde
Username

Dr. Jean-Martin Lapointe

Verified customer

投稿 Jul 13 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step 10% serum, 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (Brown adipocytes from supraclavicular fat tissue)
Specification Brown adipocytes from supraclavicular fat tissue
Permeabilization Yes - 0.3% Triton X100
Fixative Paraformaldehyde
Username

Mrs. Lena Appelsved

Verified customer

投稿 Nov 21 2013

1-10 of 26 Abreviews or Q&A

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