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RabMAb

Anti-UCH37 抗体 [EPR4896] (ab124931)

製品の概要

  • 製品名
    Anti-UCH37 antibody [EPR4896]
    UCH37 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4896] to UCH37
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cytmore details
    適用なし: ICC
  • 種交差性
    交差種: Rat, Human
    非交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to residues in Human UCH37.

  • ポジティブ・コントロール
    • Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for ICC.
  • ターゲット情報

    • 機能
      Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
    • 配列類似性
      Belongs to the peptidase C12 family.
    • 細胞内局在
      Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • UCH37 antibody
      • AD-019 antibody
      • CGI 70 antibody
      • INO80 complex subunit R antibody
      • INO80R antibody
      • Ubiquitin C terminal hydrolase L5 antibody
      • Ubiquitin C terminal hydrolase UCH37 antibody
      • Ubiquitin C-terminal hydrolase UCH37 antibody
      • Ubiquitin carboxyl terminal esterase L5 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L5 antibody
      • Ubiquitin carboxyl terminal hydrolase L5 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L5 antibody
      • Ubiquitin thioesterase L5 antibody
      • Ubiquitin thiolesterase L5 antibody
      • UCH L5 antibody
      • UCH-L5 antibody
      • UCHL5 antibody
      • UCHL5_HUMAN antibody
      see all

    画像

    • All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution

      Lane 1 : Human fetal kidney cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : NCCIT cell lysates
      Lane 4 : SH-SY5Y cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 38 kDa
    • ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Randles L  et al. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem 291:8773-83 (2016). Read more (PubMed: 26907685) »

    See 1 Publication for this product

    レビューと Q&A

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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