Anti-UCH37 抗体 [EPR4896] (ab124931)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4896] to UCH37
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UCH37 antibody [EPR4896]
UCH37 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4896] to UCH37 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Rat非交差種: Mouse -
免疫原
Synthetic peptide within Human UCH37 aa 1-100. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4896 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124931の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
ターゲット情報
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機能
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. -
配列類似性
Belongs to the peptidase C12 family. -
細胞内局在
Cytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 51377 Human
- Entrez Gene: 360853 Rat
- Omim: 610667 Human
- SwissProt: Q9Y5K5 Human
- Unigene: 591458 Human
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別名
- UCH37 antibody
- AD-019 antibody
- CGI 70 antibody
see all
画像
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All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution
Lane 1 : Human fetal kidney cell lysates
Lane 2 : 293T cell lysates
Lane 3 : NCCIT cell lysates
Lane 4 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab124931 は 10 報の論文で使用されています。
- Hameed DS et al. Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor. Front Mol Biosci 9:866467 (2022). PubMed: 35720124
- Davies CW et al. Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W. Nat Commun 12:4608 (2021). PubMed: 34326324
- Xiao M et al. MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma. Clin Transl Med 10:e241 (2020). PubMed: 33377647
- Liao Y et al. USP10 modulates the SKP2/Bcr-Abl axis via stabilizing SKP2 in chronic myeloid leukemia. Cell Discov 5:24 (2019). PubMed: 31044085
- Sha B et al. Deubiquitylatinase inhibitor b-AP15 induces c-Myc-Noxa-mediated apoptosis in esophageal squamous cell carcinoma. Apoptosis 24:826-836 (2019). PubMed: 31342239
- Elu N et al. Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination. Front Physiol 10:534 (2019). PubMed: 31130875
- Jiang L et al. Proteasomal cysteine deubiquitinase inhibitor b-AP15 suppresses migration and induces apoptosis in diffuse large B cell lymphoma. J Exp Clin Cancer Res 38:453 (2019). PubMed: 31694672
- Liao Y et al. Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene 37:1896-1910 (2018). Human . PubMed: 29353883
- Lee JH et al. Inactivation of USP14 Perturbs Ubiquitin Homeostasis and Delays the Cell Cycle in Mouse Embryonic Fibroblasts and in Fruit Fly Drosophila. Cell Physiol Biochem 47:67-82 (2018). PubMed: 29763934
- Randles L et al. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem 291:8773-83 (2016). PubMed: 26907685