製品の概要

  • 製品名
    Anti-UBXN6 antibody [5C3-1]
    UBXN6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5C3-1] to UBXN6
  • 特異性
    ab80659 recognizes both isoforms of UBXN6
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Recombinant Human UBXN6 purified from bacteria.

  • ポジティブ・コントロール
    • U2OS cotransfected with pBabepuro and pSuper based plasmid encoding anti UBXD1 shRNA

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 0.5% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    5C3-1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ICC/IF
  • 追加情報
    ICC/IF: Use at a concentration of 0.05 - 0.5 µg/ml.
    IP: Use at 1-2µg/500 3µg protein extract.
    WB: Use at a concentration of 0.3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.
    • 組織特異性
      Enhanced expression in testis.
    • 配列類似性
      Contains 1 PUB (PUG) domain.
      Contains 1 UBX domain.
    • ドメイン
      The UBX domain lacks key residues critical for VCP binding.
    • 細胞内局在
      Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • UBX domain containing 1 antibody
      • UBX domain containing 2 antibody
      • UBX domain containing protein 1 antibody
      • UBX domain containing protein 6 antibody
      • UBX domain protein 6 antibody
      • UBX domain-containing protein 1 antibody
      • UBX domain-containing protein 6 antibody
      • UBXD1 antibody
      • UBXDC2 antibody
      • Ubxn6 antibody
      • UBXN6_HUMAN antibody
      see all

    画像

    • Lanes 1 - 2 : Anti-UBXN6 antibody [5C3-1] (ab80659) at 0.3 µg/ml (in PBS/Tween)
      Lanes 3 - 4 : Ponceau S Red

      Lane 1 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 2 : U2OS were co-transfected with mismatch control
      Lane 3 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 4 : U2OS were co-transfected with mismatch control


      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 49 kDa (possible isoform).A picture of the Ponceau S stained blot after processing is shown to demonstrate equivalent loading of protein.

    参考文献

    ab80659 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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