製品の概要

  • 製品名Anti-UBXN6 antibody [5C3-1]
    UBXN6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5C3-1] to UBXN6
  • 特異性ab80659 recognizes both isoforms of UBXN6
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Recombinant Human UBXN6 purified from bacteria.

  • ポジティブ・コントロール
    • U2OS cotransfected with pBabepuro and pSuper based plasmid encoding anti UBXD1 shRNA

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 0.5% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名5C3-1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 0.05 - 0.5 µg/ml.
    IP: Use at 1-2µg/500 3µg protein extract.
    WB: Use at a concentration of 0.3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.
    • 組織特異性Enhanced expression in testis.
    • 配列類似性Contains 1 PUB (PUG) domain.
      Contains 1 UBX domain.
    • ドメインThe UBX domain lacks key residues critical for VCP binding.
    • 細胞内局在Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • UBX domain containing 1 antibody
      • UBX domain containing 2 antibody
      • UBX domain containing protein 1 antibody
      • UBX domain containing protein 6 antibody
      • UBX domain protein 6 antibody
      • UBX domain-containing protein 1 antibody
      • UBX domain-containing protein 6 antibody
      • UBXD1 antibody
      • UBXDC2 antibody
      • Ubxn6 antibody
      • UBXN6_HUMAN antibody
      see all

    Anti-UBXN6 antibody [5C3-1] 画像

    • Lanes 1 - 2 : Anti-UBXN6 antibody [5C3-1] (ab80659) at 0.3 µg/ml (in PBS/Tween)
      Lanes 3 - 4 : Ponceau S Red

      Lane 1 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 2 : U2OS were co-transfected with mismatch control
      Lane 3 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 4 : U2OS were co-transfected with mismatch control


      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 49 kDa (possible isoform).
      A picture of the Ponceau S stained blot after processing is shown to demonstrate equivalent loading of protein.

    Anti-UBXN6 antibody [5C3-1] (ab80659) 使用論文

    ab80659 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab80659.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"