The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 10 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for WB.
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions.
Protein modification; protein ubiquitination.
Belongs to the UBR4 family. Contains 1 UBR-type zinc finger.
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility.
Detection of Human UBR4 by Western blot of Immunoprecipitate.
UBR4 was immunoprecipitated from HeLa whole cell lysate, using ab114109 at 10 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-UBR4 antibody was used for the subsequent WB at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.