Anti-UBR4/p600 抗体 (ab114109)
Key features and details
- Rabbit polyclonal to UBR4/p600
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UBR4/p600 antibody
UBR4/p600 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBR4/p600 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Dog, Turkey, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Orangutan -
免疫原
Synthetic peptide corresponding to Human UBR4/p600 aa 150-200.
Database link: NP_065816.1 -
ポジティブ・コントロール
- HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab114109 was affinity purified using an epitope specific to UBR4/p600 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114109の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IP
Use at 10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the UBR4 family.
Contains 1 UBR-type zinc finger. -
細胞内局在
Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility. - Information by UniProt
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参照データベース
- Entrez Gene: 23352 Human
- Entrez Gene: 69116 Mouse
- Entrez Gene: 313658 Rat
- Omim: 609890 Human
- SwissProt: Q5T4S7 Human
- SwissProt: A2AN08 Mouse
- SwissProt: Q2TL32 Rat
- Unigene: 148078 Human
see all -
別名
- 600 kDa retinoblastoma protein associated factor antibody
- 600 kDa retinoblastoma protein-associated factor antibody
- E3 ubiquitin-protein ligase UBR4 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4/p600 with ab114109 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human UBR4/p600 by Western blot of Immunoprecipitate.
UBR4/p600 was immunoprecipitated from HeLa whole cell lysate, using ab114109 at 10 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-UBR4/p600 antibody was used for the subsequent WB at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab114109 は 1 報の論文で使用されています。
- Rinschen MM et al. The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes. Hum Mol Genet 25:1328-44 (2016). PubMed: 26792178